Human Interleukin 1 Receptor-Like 1, IL1RL1 ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human IL1RL1 ELISA kit is for the quantitative determination of human IL1RL1.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Capture Antibody: 1.0 mg/mL of mouse anti-IL1RL1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating.
2. Detection Antibody: 0.5 mg/mL ofrabbit anti-IL1RL1 monoclonal antibody conjugated to horseradish-peroxidase(HRP). Dilute to a working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard: Each vial contains 55ng of recombinant IL1RL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.

Storage

Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

12.5 pg/mL

Reconstitution And Storage

Bring all reagents to room temperature before use.
##Capture Antibody: ##1.0 mg/ml of mouse anti-IL1RL1 monoclonal antibody. Dilute to a working concentration of 2.0μg/ml in CBS before coating.
##Detection Antibody: ##0.5 mg/ml of rabbit anti-IL1RL1 monoclonal antibody conjugated to horseradish-peroxidase(HRP). Dilute to a working concentration of 0.5 μg/ml in detection antibody dilution buffer before use.
##Standard: ##Each vial contains 55ng of recombinant IL1RL1. Reconstitute with 1ml detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/ml is recommended.