Sample
cell culture supernatants, plasma, and serum samples.
Intended Use
The Human IL-6 sR (interleukin 6 soluble receptor) ELISA kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human IL-6 sR in serum, plasma, and cell culture supernatants.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.
Precision
Intra-Assay CV%: <10%
Inter-Assay CV%: <12%
Sensitivity
The minimum detectable dose of Human IL-6 sR was determined to be 3 pg/ml.
Minimum detectable dose is defined as the analyte concentration resulting in an absorbance that is 2 standard deviations higher than that of the blank (diluent buffer).
Standard Curve
These standard curves are for demonstration only. A standard curve must be run with each assay.
Citations
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Chalaris, A; Schmidt-Arras, D; et al. Interleukin-6 Trans-Signaling and Colonic Cancer Associated with Inflammatory Bowel Disease. DIGESTIVE DISEASES 30:492-499(2012).
Okada, SK; Fischer, R; et al. Peripheral B cell abnormalities in systemic lupus erythematosus: Comparative phenotypes assessed by polychromatic flow cytometry and effects of treatment with humanized anti-IL6R monoclonal antibody.. ARTHRITIS AND RHEUMATISM 50:4081-4081(2004).
COA
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