Intended Use
Human IAA ELISA Kit is an enzyme immunoassay for the quantitative determination of IgG autoantibodies and antibodies to insulin in human serum.
Contents of Kit
1. Microtiter plate: 12 breakable strips, 8 wells per strip, coated with human recombinant insulin (vacuum sealed with desiccant)
2. Concentrated wash buffer: sufficient for 1000 ml (100 ml, concentrate, white capped)
3. Anti human IgG (sheep) Horser-peroxidase (HRP): complex (15ml, ready for use, red capped)
4. Substrate: 3,3´,5,5´-Tetramethylbenzidin (15 ml, ready for use, blue capped)
5. Stop solution: 0.25 M sulfuric acid (15 ml, ready for use, yellow capped)
6. Sample diluent (100 ml, ready for use, black capped)
7. positive control: concentration see leaflet (1 ml, ready for use, red capped)
8. Calibrators: concentrations see leaflet (5 vials, 1 ml each, ready for use, white capped)
Storage
The expiry date of each component is reported on its respective label, that one of the complete Human IAA ELISA Kit on the box label. Upon receipt, all components of the Human IAA ELISA Kit have to be kept at 2 - 8 °C, preferably in the original Human IAA ELISA Kit box.
Performance Characteristics
Calibration: The Human IAA ELISA Kit is artificially calibrated and concentrations of IAA are therefore expressed in U/ml.
Detection Limit
The analytical sensitivity (lower detection limit, 0 + 3 SD) was established to be 0.08 U/ml. The functional sensitivity was measured as 20 % of interassay CV at 0.9 U/ml.
Standard Curve
Do not use for evaluation!
Citations
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COA
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