serum, plasma, adipocyte extracts, culture media samples
The Human High Molecular Weight (HMW) Adiponectin ELISA kit is intended for the non- radioactive quantification of Human HMW Adiponectin in serum, plasma, and adipocyte extracts or culture media samples with a simple sample pretreatment.
Contents of Kit
A. Human HMW Adiponectin ELISA Plate: 1 Strip plate
B. Adhesive Plate Sealer Quantity: 2 sheets
C. 10X HRP Wash Buffer Concentrate: 2 bottles containing 50 mL each
D. Human HMW Adiponectin Standard: 0.5 mL upon hydration.
E. Human HMW Adiponectin Digestion Controls 1 and 2 One vial: 0.25 mL/bottle, Lyophilized
F. Sample Digestion Solution: 600 μL
G. Sample Digestion Buffer: 8 mL
H. Sample Dilution Buffer (10x): 1 mL
I. 10x Assay Buffer: 50 mL (10x)
J. Assay Running Buffer: 13 mL
K. Sample Preparation Plates: Two 96-well solid plates with 2 plate sealers
L. Human HMW Adiponectin Detection Antibody Pre-titered Biotinylated Polyclonal anti-Adiponectin Antibody: 12 mL
M. Enzyme Solution: 12 mL
N. Substrate: 12 mL
O. Stop Solution: 12 mL
Recommended storage for kit components is 2-8°C.
All components are shipped and stored at 2-8°C. Reconstituted standards and controls can be frozen for future use but repeated freeze thaws should be avoided. Refer to expiration dates on all reagents prior to use. Do not mix reagents from different kits unless they have the same lot numbers.
Intra-assay Variation: 0.97%-3.41%
Inter-assay Variation: 3.01%-9.10%