Human Follicular Dendritic Cell Secreted peptide (C4orf7) ELISA Kit

Sample

Serum, plasma, tissue homogenates

Species Reactivity

Human

Detection Method

iELISA

Intended Use

For the quantitative determination of human follicular dendritic cell secreted peptide (C4orf7) concentrations in serum, plasma, tissue homogenates.

Contents of Kit

1. Assay plate (12 × 8 coated Microwells), 1(96 wells)
2. Standard (Freeze dried), 2
3. Biotin-antibody (100× concentrate), 1 × 120 μl
4. HRP-avidin (100× concentrate), 1 × 120 μl
5. Biotin-antibody Diluent, 1 × 15 ml
6. HRP-avidin Diluent, 1 × 15 ml
7. Sample Diluent, 1 × 50 ml
8. Wash Buffer (25× concentrate), 1 × 20 ml
9. TMB Substrate, 1 × 10 ml
10. Stop Solution, 1 × 10 ml
11. Adhesive Strip (For 96 wells), 4
12. Instruction manual, 1

Storage

Unopened kit: Store at 2 - 8°C. Do not use the kit beyond the expiration date.
Opened kit:
1. Coated assay plate: May be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.
2. Standard: May be stored for up to 1 month at 2 - 8° C. If don' t make recent use, better keep it store at -20°C.
3. Biotin-antibody: May be stored for up to 1 month at 2 - 8° C. If don' t make recent use, better keep it store at -20°C.
4. HRP-avidin: May be stored for up to 1 month at 2 - 8° C. If don' t make recent use, better keep it store at -20°C.
5. Biotin-antibody Diluent: May be stored for up to 1 month at 2 - 8°C.
6. HRP-avidin Diluent: May be stored for up to 1 month at 2 - 8°C.
7. Sample Diluent: May be stored for up to 1 month at 2 - 8°C.
8. Wash Buffer: May be stored for up to 1 month at 2 - 8°C.
9. TMB Substrate: May be stored for up to 1 month at 2 - 8°C.
10. Stop Solution: May be stored for up to 1 month at 2 - 8°C.
*Provided this is within the expiration date of the kit

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

7.81 ng/ml-500 ng/ml

Sensitivity

The minimum detectable dose of human C4orf7 is typically less than 1.95 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.