Human Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32), FCGR2B ELISA Kit (CKERS-FCGR2B-232H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5plates
Species Reactivity
Human
Intended Use
The human FCGR2B ELISA kit is for the quantitative determination of human FCGR2B.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-FCGR2B monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
3. Detection Antibody: 0.6 mg/mL of biotinylated rabbit anti-FCGR2B polyclonal antibody. Dilute to a working concentration of 1 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 15 ng of recombinant FCGR2B. Reconstitute with 1 mL detection antibody dilution buffer. A six-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
5. Streptavidin-HRP: 50μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.
Storage
Keep streptavidin-HRP at 4°C and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
The minimum detectable dose of human FCGR2B was determined to be approximately 31.25 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

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References


FCGR2B and FCRLB Gene Polymorphisms Associated with IgA Nephropathy

PLOS ONE

Authors: Zhou, Xu-jie; Cheng, Fa-juan; Qi, Yuan-yuan; Zhao, Yan-feng; Hou, Ping; Zhu, Li; Lv, Ji-cheng; Zhang, Hong

Background: IgA nephropathy (IgAN) is a complex syndrome characterized by deposition of IgA and IgA containing immune complexes (ICs) composed of IgG and complement C3 proteins in the mesangial area of glomeruli. The low-affinity receptors for the Fc region of IgG (Fc gamma Rs) are involved in autoantibody/immune complex-induced organ injury as well as ICs clearance. The aim of the study was to associate multiple polymorphisms within FCGR gene locus with IgAN in a large Chinese cohort. Patients and Methods: 60 single nucleotide polymorphisms (SNPs) spanning a 400 kb range within FCGR gene locus were analyzed in 2100 DNA samples from patients with biopsy proven IgAN and healthy age- and sex-matched controls from the same population in Chinese. Results: Among the 60 SNPs investigated, 15 gene polymorphisms within FCGR gene locus (25%) were associated with susceptibility to IgAN. The most significantly associated SNPs within individual genes were FCGR2B rs12118043 (p = 8.74*10(-3), OR 0.76, 95% CI 0.62-0.93), and FCRLB rs4657093 (p = 2.28*10(-3), OR 0.77, 95% CI 0.65-0.91). Both conditional analysis and linkage disequilibrium analysis suggested they were independent signals associated with IgAN. Associations between FCGR2B rs12118043 and proteinuria (p = 3.65 x 10(-2)) as well as gross hematuria (p = 4.53 x 10(-2)), between FCRLB rs4657093 and levels of serum creatinine (p = 2.67 x 10(-2)) as well as eGFR (p = 5.41*10(-3)) were also observed. Electronic cis-expression quantative trait loci analysis supported their possible functional significance, with protective genotypes correlating lower gene expressions. Conclusion: Our data from genetic associations and expression associations revealed potentially pathogenic roles of Fc receptor gene polymorphisms in IgAN.

The functional variant of the inhibitory Fc gamma receptor IIb (CD32B) is associated with the rate of radiologic joint damage and dendritic cell function in rheumatoid arthritis

ARTHRITIS AND RHEUMATISM

Authors: Radstake, Timothy R. D. J.; Franke, Barbara; Wenink, Mark H.; Nabbe, Karin C. A. A.; Coenen, Marieke J. H.; Welsing, Paco; Bonvini, Ezio; Koenig, Scott; van den Berg, Wim B.; Barrera, Pilar; van Riel, Piet L. C. M.

Objective. Fc gamma receptors (Fc gamma Rs) recognize immune complexes (ICs) and coordinate the immune response by modulating the functions of dendritic cells (DCs). The purpose of this study was to unravel the role of the inhibitory Fc gamma RIIb in rheumatoid arthritis (RA) by studying the effect of the FCGR2B 695T > C polymorphism on susceptibility to RA, severity of the disease, and DC function. Methods. Genotyping was performed in RA patients (n = 246) and healthy blood donors (n = 269). The patients' demographic data, disease severity, and disease progression were assessed over a followup of 6 years. DCs were cultured for flow cytometry to determine the expression of Fc gamma Rs. For detection of Fc gamma RIIb (CD32B), a unique anti-Fc gamma RIIb antibody (2B6-fluorescein isothiocyanate [FITC]) was used. The capacity for antigen uptake by DCs was studied by assessing the uptake of FITC-labeled ICs. Levels of cytokine production by DCs were measured during lipopolysaccharide-mediated cell activation in the presence and absence of ICs. Results. Although no role of the FCGR2B variant in RA susceptibility was demonstrated, this variant was associated with a nearly doubled rate of radiologic joint damage during the first 6 years of RA. Multiple regression analysis showed that FCGR2B was by far the strongest predictor of joint damage identified to date. DCs from patients carrying this variant failed to display the inhibitory phenotype normally observed upon IC-mediated triggering of inflammation and displayed diminished Fc gamma RII-mediated antigen uptake compared with wild-type DCs. However, the levels of Fc gamma Rs were not affected, suggesting that the FCGR2B variant alters the function rather than regulation of proteins. Conclusion. This study is the first to show that a single genetic variant, the FCGR2B 695T>C polymorphism, is a critical determinant of disease severity in RA and radically changes DC behavior. Our results underscore the key role of DCs in the progression of RA and reveal Fc gamma RIIb as an important potential therapeutic target in RA and other autoimmune conditions.

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