Human Epithelial Cell Adhesion Molecule, EPCAM ELISA Kit (CKERS-EPCAM-240H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5plates
Species Reactivity
Human
Intended Use
The human EPCAM ELISA kit is for the quantitative determination of human EPCAM.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.2 mg/mL of mouse anti-EPCAM monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
3. Detection Antibody: 0.5 mg/mL of rabbit anti-EPCAM monoclonal antibody conjugated to horseradish-peroxidase(HRP). Dilute to a working concentration of 1 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 50ng of recombinant EPCAM. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3,000 pg/mL is recommended.
Storage
Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
The minimum detectable dose of human EPCAM was determined to be approximately 46.8 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

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References


Raymond, VM; Morris, AM; et al. MLH1 promotor hypermethylation does not rule out a diagnosis of Lynch syndrome: a case report. FAMILIAL CANCER 14:77-80(2015).
Gao, P; Pinkston, KL; et al. Deglycosylation of mAb by EndoS for Improved Molecular Imaging. MOLECULAR IMAGING AND BIOLOGY 17:195-203(2015).

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