Human Dickkopf Homolog 3 (Xenopus laevis), DKK3 ELISA Kit (CKERS-DKK3-239H)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
5plates
Species Reactivity
Human
Intended Use
The human DKK3 ELISA kit is for the quantitative determination of human DKK3.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-DKK3 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
3. Detection Antibody: Each vial contains 120μg biotinylated rabbit anti-DKK3 polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 98 ng of recombinant DKK3. Reconstitute standard power with sterile distilled water. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4,000 pg/mL is recommended.
5. Streptavidin-HRP: 50μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.
Storage
Keep streptavidin-HRP at 4°C and protect it from pro-longed exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
The minimum detectable dose of human DKK3 was determined to be approximately 62.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Citations


Have you cited CKERS-DKK3-239H in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


Chu, QQ; Cai, LY; et al. Dkk2/Frzb in the dermal papillae regulates feather regeneration. DEVELOPMENTAL BIOLOGY 387:167-178(2014).
Papatriantafyllou, M; Moldenhauer, G; et al. Dickkopf-3, an immune modulator in peripheral CD8 T-cell tolerance. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109:1631-1636(2012).

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:
Verification code
Click image to refresh the verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket