Human Cathepsin L ELISA Kit (DEIA7176)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, other biological fluids, cell culture supernatants
Species Reactivity
Human
Intended Use
The Human Cathepsin L ELISA Kit is intended for quantitation of human cathepsin L in body fluids like serum or in cell culture supernatants.
Contents of Kit
1. Cathepsin L Monoclonal Antibody Coated 96 Plate
2. Biotin-Conjugate Concentrate
3. Streptavidin-HRP
4. Cathepsin L Standard
5. 20X Wash Buffer Concentrate
6. 20X assay buffer concentrate
7. Sample Diluent
8. Substrate Solution
9. Stop Solution
10. Blue-dye
11. Green-dye
12. Red-dye
Storage
Upon arrival, store the entire contents of the kit at 4°C. Following initial use, all components should continue to be stored at 4°C.
Detection Range
3-50 ng/mL
Sensitivity
≤1.7 ng/mL

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References


A humanized antibody inhibitor for cathepsin L

PROTEIN SCIENCE

Authors: Shi, Xiaojing; Zhang, Yong

Cathepsin L (CTSL) is a cysteine protease involved in a variety of physiological and pathological processes. Potent inhibitors against CTSL have long been sought for drug development. Due to insufficient specificity and suboptimal pharmacological properties for current CTSL inhibitors, novel agents are still required for selectively blocking CTSL activity. Here we generated a humanized antibody inhibitor of CTSL by genetically fusing the inhibitory propeptide of procathepsin L to the N-terminus of the light chain of a humanized antibody. The resulting antibody fusion could be stably expressed and displays highly potent inhibition activity and specificity toward CTSL. This work demonstrates a new approach for the rapid generation of antibody inhibitors of CTSL. It can possibly be extended to create inhibitory antibodies targeting other cathepsin proteases, providing novel research and therapeutic tools.

Nonmetal species in the carbon modified TiO2 and its visible light photocatalytic activity

APPLIED SURFACE SCIENCE

Authors: Shi, Yanfen; Chen, Feng; Zhang, Jinlong

A carbon modified TiO2 (CT) was synthesized by hydrolyzing titanium tetrachloride with diethylamine and calcination at 400 degrees C. CT was then handled with a NaOH aqueous solution elution and a subsequent re-assembling treatment. X-ray diffraction (XRD), transmission electron microscopy (TEM), nitrogen adsorption-desorption analysis, X-ray photoelectron spectroscopy (XPS), thermogravimetric and differential thermal analysis (TG-DTA), chemical oxygen demand (COD) and UV-vis diffuse reflectance spectroscopy (DRS) were then used to assess the changes of CT during the whole process. It is revealed that carbon in the CT should mostly be presented as surface deposited organic matters but not likely doped into the TiO2 lattice. CT exhibits obvious visible absorption and high photocatalytic activity for the degradation of 2,4-dichlorophenol (DCP) under visible light irradiation. Meanwhile, CT photocatalyst possesses excellent stability and reusability. NaOH solution elution washes off a large amount of surface deposited organics and worsens the visible absorbance and photocatalytic activity of CT, which can be well recovered by the re-assembling treatment. The re-assembled photocatalyst, CTSL, exhibits exhibits a very similar photocataytic activity with CT for degradation of DCP under the visible light irradiation, but is much higher than that of CTS. (C) 2012 Elsevier B.V. All rights reserved.

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