HUMAN CTRP4 ELISA KIT (DEIA6967)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Serum, EDTA Plasma
Species Reactivity
Human
Intended Use
Human CTRP4 Immunoassay is a 3.5 - 4.5 hour solid phase ELISA designed to measure Human CTRP4 in cell culture supernates, serum and plasma.
Contents of Kit
1. CTRP4 Microplate
2. CTRP4 Standard
3. Detection Antibody
4. Positive Control
5. Anti Rabbit IgG-HRP
6. Dilution Buffer
7. Wash Buffer
8. TMB Substrate Solution
9. Stop Solution
10. Plate Sealer
Storage
2 - 8°C
Precision
Intra-assay Precision: 6-8%
Inter-assay Precision: 10-12%
Detection Range
1.56-100 ng/mL
Sensitivity
0.78 ng/mL
General Description
This Human CTRP4 ELISA Kit contains the necessary components required for the quantitative measurement of recombinant and/or natural human CTRP4 from serum and plasma in a sandwich ELISA format. This immunoassay contains recombinant human CTRP4 and antibodies raised against this protein. Results from this immunoassay have shown to accurately quantify recombinant and natural CTRP4 samples.
Reconstitution And Storage
Unopened Kit: Store at 2-8°C for up to 6 months. For longer storage, unopened Standard, Positive Control and Detection Antibody Concentrate should be stored at -20°C or -70°C. Do not use kit past expiration date.
Opened/Reconstituted Reagents: Reconstituted Standard (stock) solution and Detection Antibody concentrated solution SHOULD BE STORED at -20°C or -70°C for up to one month. Anti Rabbit IgG-HRP Conjugate 100-fold concentrated solution (protectfrom light) and other components may be stored at 2 - 8°C for up to 6 months. Do not freeze Substrate Solution and HRP.
Microplate Wells: Return unused wells to the plastic pouch with the desiccant pack. Microplate may be stored for up to 6 months at 2 - 8°C.

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References


Li, JM; Zhang, XQ; et al. Temporal evolution of gene expression in rat carotid artery following balloon angioplasty. JOURNAL OF CELLULAR BIOCHEMISTRY 101:399-410(2007).
Chacko, SA; Sul, J; et al. Magnesium supplementation, metabolic and inflammatory markers, and global genomic and proteomic profiling: a randomized, double-blind, controlled, crossover trial in overweight individuals. AMERICAN JOURNAL OF CLINICAL NUTRITION 93:463-473(2011).

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