This Human CTRP3 ELISA Kit contains the necessary components required for the quantitative measurement of recombinant and/or natural human CTRP3 from serum and plasma in a sandwich ELISA format.
This immunoassay contains recombinant human CTRP3 and antibodies raised against this protein. Results from this immunoassay have shown to accurately quantify recombinant and natural CTRP3 samples.
Contents of Kit
1. CTRP3 Microplate - 96 well polystyrene microplate (12 strips of 8 wells) coated with antibody against CTRP3. 1 plate
2. CTRP3 Standard - 320 ng/vial of recombinant human CTRP3 in a buffered protein base with preservative; lyophilized. 1 vial
3. Detection Antibody Concentrate - 1.2 mL/vial, 10-fold concentrate of an antibody against CTRP3 with preservative; lyophilized. 1 vial
4. Positive Control - one vial of recombinant human CTRP3; lyophilized. 1 vial
5. Streptavidin-HRP Conjugate - 120 μL/vial, 100-fold concentrated solution of Goat anti rabbit IgG conjugate to HRP. 1 vial
6. Dilution Buffer - 60 mL of buffered protein based solution with preservative. 1 bottle
7. Wash Buffer - 50 mL of 10 - fold concentrated buffered surfactant, with preservative. 1 bottle
8. TMB Substrate Solution - 11 mL of TMB substrate solution. 1 bottle
9. Stop Solution - 11 mL of 0.5M HCI. 1 bottle
10. Plate Sealer: 1
11. Plastic Pouch: 1
Unopened Kit: Store at 2-8°C for up to 8 months. For longer storage, unopened Standard, Positive Control and Detection Antibody Concentrate should be stored at -20°C or -70°C. Do not use kit past expiration date.
Opened/Reconstituted Reagents: Reconstituted Standard (stock) solution and Detection Antibody concentrated solution SHOULD BE STORED at -20°C or -70°C for up to one month. ARIGHRP Conjugate 100-fold concentrated solution and TMB Substrate Solution can be stored at 2-8°C for up to 8 months (DO NOT FREEZE and PROTECT FROM LIGHT). All other components may be stored at 2-8°C for up to 8 months.
Microplate Wells: Return unused strips to the plastic pouch with the desiccant pack. Microplate may be stored for up to 6 months at 2-8°C after opening.
Intra-assay Precision (CV%): 4 - 6%
Inter-assay Precision (CV%): 8 - 10%