Human CD200 Molecule, CD200 ELISA Kit (CKERS-CD200-230H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5plates
Species Reactivity
Human
Intended Use
The human CD200 ELISA kit is for the quantitative determination of human CD200.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-CD200 monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
3. Detection Antibody: 0.5 mg/mL of biotinylated rabbit anti-CD200 polyclonal antibody. Dilute to a working concentration of 2 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 100 ng of recombinant CD200. Reconstitute with 1 mL detection antibody dilution buffer.After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
5. Streptavidin-HRP: 50μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.
Storage
Keep streptavidin-HRP at 4°C and protect it from pro-longed exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
The minimum detectable dose of human CD200 was determined to be approximately 7.8 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

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