Association of Exosomal miR-34a with Markers of Dyslipidemia and Endothelial Dysfunction in Children and Adolescents with T1DM
JOURNAL OF CLINICAL RESEARCH IN PEDIATRIC ENDOCRINOLOGY
Authors: Ibrahim, Alshaymaa A.; Wahby, Aliaa A.; Ashmawy, Ingy; Saleh, Rehan M.; Soliman, Hend
Objective: Dyslipidemia and endothelial dysfunction are common disorders and major causative factors for atherosclerosis in patients with type I diabetes mellitus (T1DM). However, their pathophysiology in young patients with T1DM is still under evaluated. We aimed, for the first time, to assess the expression of exosomal micro-RNA 34a (miR-34a) in serum of children and adolescents with T1DM and correlate this expression with markers of dyslipidemia and endothelial dysfunction. Methods: The study included 120 T1DM patients and 100 control subjects. Assessment of miR-34a was performed using quantitative real-time polymerase chain reaction. Lipid profile was assessed on an automated analyzer and serum endoglin and intracellular adhesion molecule (ICAM) concentrations were measured using immunometric methods. Results: Relative expression of miR-34a and serum endoglin and ICAM concentrations were higher in patients than controls (p = 0.001) and in patients with dyslipidemia (42 patients) compared to patients without dyslipidemia (78 patients) (p = 0.01). Linear regression analysis revealed a strong independent association between exosomal miR-34a expression and total cholesterol, low-density lipoprotein, serum endoglin and serum ICAM after adjustment for other cofactors. The utility of miR-34a as an indicator for associated dyslipidemia was tested using receiver operator characteristic curve analysis which revealed area under the curve: 0.73 with confidence interval: 0.63-0.83 and p = 0.001. Conclusion: This was the first study to show the altered expression of exosomal miR-34a among children and adolescents with T1DM. Moreover, association of miR-34a with markers of dyslipidemia and endothelial dysfunction was identified, suggesting that it could play a role in regulation of lipid metabolism and endothelial function in T1DM.
Effect of dietary orange and grapefruit peel on growth performance, health status, meat quality and intestinal microflora of broiler chickens
ITALIAN JOURNAL OF ANIMAL SCIENCE
Authors: Vlaicu, Petru Alexandru; Untea, Arabela Elena; Panaite, Tatiana Dumitra; Turcu, Raluca Paula
In our experiment we studied the effect of dietary orange peel (OP) and grapefruit peel (GP) on broilers health and thigh meat quality. The experiment was conducted on 126 Cobb 500 broiler chicks, assigned to three dietary treatments with 42 broilers per group. Each treatment was conducted in 3 experimental growth boxes, corresponding to 6 replicate pens per treatment and 7 broilers per pen. The growth boxes had identical size with a capacity of 3.5 m(2) per box. Experimental dietary treatments differed from control diet (C) by addition of 2% OP or 2% GP supplement. A significant increase in body weight was exhibited in OP group compared to GP group (p < .05). Serum energetic profile (glucose, cholesterol and triglyceride) were lowered in both OP and GP supplemented groups compared to C birds (p < .05). Among fatty acids in thigh meat, OP group has increased the sum of PUFA compared to GP group. Dietary OP and GP have effectively reduced the oxidation process occurring during storage measured by the thiobarbituric acid-reactive substances (TBARS) values in thigh meat. Therefore, lipid oxidation was delayed by incorporating the two citrus peels in broiler diet. Monitoring the antimicrobial effect of OP and GP on intestinal and caecal microbiota population, the supplements reduced the growth of pathogenic Escherichia coli and Staphylococcus spp., proving their antimicrobial effect, while the beneficial bacteria, Lactobacillus spp. was significantly improved.