Human C-Reactive Protein, Pentraxin-Related, CRP ELISA Kit (DEIA217)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, other biological fluids
Species Reactivity
Human
Intended Use
This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human ADIPOQ in serum, plasma and other biological fluids.
Contents of Kit
1. Human ADIPOQ Microplate: polystyrene Microplate coated with a monoclonal antibody against Human ADIPOQ
2. Standard (freeze dried): 50 ng/mL, 2 vials
3. Biotin-antibody (100x): 2 vials
4. HRP-avidin (100x): 2 vials
5. Reagent Diluent: 25 mL, 2 vials
6. TMB Substrate: 12 mL, 1 vial
7. TMB Stop Solution: 1 vial
8. Wash Buffer (20X): 30 mL, 1 vial
Storage
Store components of the kit at 2-8°C upon arrival up to the expiration date. For more detailed information, please download the following document on our website.

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References


High-Sensitive c-Reactive Protein Levels in Euthymic Bipolar Patients: Case-Control Study

PSYCHIATRIC QUARTERLY

Authors: Hamdi, Ghada; Ammar, Hanen Ben; Khelifa, Emira; Chaaben, Arij Ben; Khouadja, Sabria; Ayari, Fayza; Mihoub, Ons; Tamouza, Ryad; Guemira, Fethi; Elhechmi, Zouhaier

Bipolar disorder is a chronic, disabling disease that is characterized by the recurrence of thymic episodes. The role of the immune-inflammatory system in the etiopathogenesis of this affection arouses the interest of research. The aim of this work was to determine the plasma levels of the high sensitivity C reactive protein (hs-CRP) in patients with bipolar disorder in remission phase by comparing them to a control group. A case-control cross-sectional study was conducted from 56 subjects with bipolar disorder in clinical remission, and 56 volunteers and healthy control subjects. Mean plasma hs-CRP was significantly higher in patients with bipolar disorder than control subjects. In bipolar patients, a hs-CRP elevation was significantly associated with the disease severity item mean score. Through this study, bipolar disorder appears to be associated with a state of chronic inflammation. This should lead to randomized controlled trials evaluating the value of anti-inflammatory drugs in the management of bipolar disorder.

Outcomes of COVID-19 in Patients With Lung Cancer Treated in a Tertiary Hospital in Madrid

FRONTIERS IN ONCOLOGY

Authors: Calles, Antonio; Inmaculada Aparicio, Maria; Alva, Manuel; Bringas, Marianela; Gutierrez, Natalia; Soto, Javier; Arregui, Marta; Clara Tirado, Victoria; Luis Alvarez, Enrique; del Monte-Millan, Maria; Massarrah, Tatiana; Galera, Mar; Alvarez, Rosa; Martin, Miguel

Background:Cancer patients represent a vulnerable population for COVID-19 illness. We aimed to analyze outcomes of lung cancer patients affected by COVID-19 in a tertiary hospital of a high-incidence region during the pandemic. Methods:We annotated 23 lung cancer patients consecutively diagnosed with COVID-19 at our institution (HGUGM; Madrid, Spain) between March 4th, 2020 and May 12th, 2020. Only patients with a confirmatory SARS-CoV-2 RT-PCR were included in the study. Results:All patients had at least 1 COVID-19 related symptom; cough (48%), shortness of breath (48%), fever (39%), and low-grade fever (30%) were the most common. Time from symptoms onset to first positive SARS-CoV-2 PCR was 5.5 days (range 1-17), with 13% of cases needed from a 2nd PCR to confirm diagnosis. There was a high variability on thoracic imaging findings, with multilobar pneumonia as the most commonly found pattern (74%). Main lab test abnormalities were low lymphocytes count (87%), high neutrophil to lymphocyte ratio -NLR- (78%), and elevated inflammatory markers: fibrinogen (91%), c-reactive protein -CRP- (87%), and D-dimer (70%). In our series, hospitalization rate was 74%, 39% of patients developed acute respiratory distress syndrome (ARDS), and the case-fatality rate was 35% (8/23). 87% of patients received anti-viral treatment (87% hydroxychloroquine, 74% lopinavir/ritonavir, 13% azithromycin), 43% corticosteroids, 26% interferon-beta, 4% tocilizumab, and 82% of hospitalized patients received anticoagulation. High-oxygen requirements were needed in 39% of patients, but only 1 pt was admitted for invasive MV and was discharged 42 days after admission. Multiple variables related to tumor status, clinical baseline conditions, and inflammation markers were associated with mortality but did not remain statistically significant in a multivariate model. In patients with lung cancer receiving systemic therapy (n= 242) incidence and mortality from COVID-19 were 4.5, and 2.1%, respectively, with no differences found by type of treatment. Conclusions:Lung cancer patients represent a vulnerable population for COVID-19, according to the high rate of hospitalization, onset of ARDS, and high mortality rate. Although larger series are needed, no differences in mortality were found by type of cancer treatment. Measures to minimize the risk of SARS-CoV-2 infection remain key to protect lung cancer patients.

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