Human Bordetella pertussis IgA ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Quantitative and qualitative tests for the detection of human antibodies in serum or plasma against Aspergillus fumigatus.

Contents of Kit

1.Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96) MTP, 1 frame, the coating material is inactivated, 12
2.Standard serum (ready-to-use) STD Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide, colouring: Amaranth O, 2 x 2 ml
3.Negative control serum (ready-to-use) NEG Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, colouring: Lissamin green V, 2 ml
4.Anti-human-IgA-conjugate (ready-to-use) APC Anti-human-IgA from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution, preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane, 13 ml
5.Washing solution concentrate (sufficient for 1 litre) WASH Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide, 33.3 ml
6.Dilution buffer DILB Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide 0.01 g/l Bromphenol blue sodium salt, 2 x 50 ml
7.Stopping solution STOP 1.2 N sodium hydroxide, 15 ml
8.Substrate (ready-to-use) pNPP Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!), 13 ml
9.Quality control certificate with standard curve and evaluation table INFO (quantification of antibodies in IU/ml or U/ml), 1

Storage

General Description

Four species, Bordetella pertussis, B. parapertussis, B. bronchiseptica and B. avium belong to the bacterial genus of Bordetella. These bacteria are ubiquitous, small (0.2 – 0.5 μm), gram-negative obligatory aerobic coccoid single or paired bacteria. The ideal temperature for reproduction is 35 - 37 °C. B. pertussis and B parapertussis are human pathogens causing whooping cough (pertussis) and a milder form, which is less common (atypical pertussis). The other two species mostly cause respiratory diseases in warmblooded animals. However, in rare cases (e.g. close contact with animals) B. bronchiseptica is an opportunistic secondary human pathogen in case of infections like bronchitis, pneumonia or wound infections. Even though there are phenotypic differences between the three human pathogenic Bordetella species, their DNA is 72-94% homologous. Therefore, it is currently being discussed whether to combine them in a single species or three subspecies of one species.
Bordetella pertussis causes whooping cough, a worldwide infectious disease that is transmitted from person to person by droplet infection. Especially children at the age of 0-4 years are affected, and the mortality of infected infants is high.
The attachment of B. pertussis to the tuft of ciliated cells in the mucosa of the human respiratory tract is mediated by adhesins. An important adhesion protein and an equally important immunogen is the so called filamentous hemagglutinin (FHA). Colonizing of the respiratory tract and establishment of infection are facilitated by the synergistic action of several virulence factors.
A significant virulence factor is pertussis toxin (PT), which is exclusively synthesized and secreted by B. pertussis. Multiple biologic effects like leukocytosis, lymphocytosis, mitogenicity and increased sensitivity to histamine are caused by PT.
The typical whooping cough can be divided into three stages: the catarrhal phase, the convulsivum (paroxysmal) phase, and the decrementi phase. The ELISA technique is the most commonly chosen method for B. pertussis antibody detection.