Human Alpha-3-HS-Glycoprotein, AHSG ELISA Kit (CKERS-AHSG-243H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5plates
Species Reactivity
Human
Intended Use
The human AHSG ELISA kit is for the quantitative determination of human AHSG.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-AHSG monoclonal antibody.
3. Detection Antibody: Each vial contains 120 μg biotinylated rabbit anti-AHSG polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 2.0 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 52 ng of recombinant AHSG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2,000 pg/mL is recommended.
5. Streptavidin-HRP: 50μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.
Storage
Keep streptavidin-HRP at 4°C and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
The minimum detectable dose of human AHSG was determined to be approximately 31.25 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

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