Human Alpha-2-HS-Glycoprotein, AHSG ELISA Kit (CKERS-AHSG-242H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5plates
Species Reactivity
Human
Intended Use
The human AHSG ELISA kit is for the quantitative determination of human AHSG.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-AHSG monoclonal antibody.
3. Detection Antibody: 0.5 mg/mL of rabbit anti-AHSG monoclonal antibody conjugated to horseradish-peroxidase(HRP). Dilute to a working concentration of 1.0 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 24 ng of recombinant AHSG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.
Storage
Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
The minimum detectable dose of human AHSG was determined to be approximately 12.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

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