HCV Nonstructural Protein 4 (aa 1658 - 1863), recombinant protein from E. coli
Hepatitis C Virus NS4 protein fragment
Endoplasmic reticulum; multi-pass membrane protein.
Reacts strongly with human HCV positive serum.
> 95 % by SDS-PAGE.
Preservative: None Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. Preservative: None Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral protease
NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
HCV; HCV NS4; Hepatitis C Virus nonstructural antigen 4; Non structural protein 4A; Non structural protein 4B; NS4A; NS4B; p27; p8