Human Haemophilus Influenza B IgG ELISA is an enzyme immunoassay for determination of IgG antibodies against polyribosylribitol phosphate of Haemophilus influenzae type B in human serum and plasma.
Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.
Haemophilus influenzae type B (HiB) is a very common cause of invasive critical infectious diseases in children up to the age of
six. Following infection the symptoms of the disease include: Pericarditis, osteomyelitis, meningitis, encephalitis, pneumonia, sinusitis and otitis. In many cases the disease is lethal or leads to neurological damage, which cannot always be prevented by rapid antibiotic therapy. The underlying reason for the disease is very often a latent immunodeficiency with a specifically reduced humoral immune response to the polyribosylribitol phosphate (PRP) in the polysaccharide encapsulation of the bacterium. In children another reason is the immaturity of the immune system. Today often the term "immunocompromised patients" is used,comprising all acquired and innate specific and unspecific immunodeficiencies.
As a result, in children of 3 months of age or older a vaccination with different sorts of PRP-containing vaccines is recommended. This can lead to a clear reduction in the number of infections with Haemophilus influenzae type B.The titer of antibodies produced by vaccination can be used to confirm whether the vaccination has been successful. The HiB IgG is used to measure the level of PRP-specific IgG-antibodies following a 4-6 week period after complete immunization to monitor the humoral immune status of children or other individuals at risk.
Monitoring of the humoral immunostatus after vaccination. Verification of the diagnosis Haemophilus influenzae type B infection by repeated monitoring of antibody concentrations. Risk assessment in immunocompromised patients leading to a failure of vaccination with a PRP-containing vaccine.
Reconstitution And Storage
The kit is shipped at ambient temperature and should be stored at 2-8 °C. Keep away from heat or direct sun light. The storage
and stability of specimen and prepared reagents is stated in the corresponding chapters.
The microtiter strips are stable up to 6 mon in the broken, but tightly closed bag when stored at 2-8°C.