Herpes Simplex Virus Type 1 (HSV-1) Antigen ELISA Kit (DEIAFY104)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
2 plate
Sample
complex sample matrices
Species Reactivity
Human
Intended Use
The test kit incorporates proprietary diluents that are designed to both lyse virions and prevent the development of nonspecific signal derived from complex sample matrix effects and/or the nonspecific adsorption of reactive test components, resulting in improvements in both sensitivity and specificity.
Contents of Kit
1. HSV-1 Antigen Capture Plate (96 tests)
2. HSV-1 Positive Control (lyophilized)
3. HSV Negative Control (lyophilized)
4. HSV-1 Detection Antibody, HRP-labeled (1X)
5. Wash Buffer (20X)
6. Sample Preparation Reagent (1X)
7. Chromagen Solution (1X)
8. Stop Solution (1X)
9. Antigen Diluent (1X)
10. Sample Dilution Tray
Storage
Store all kit components at 2-8°C. Crystal formation may occur in the wash buffer concentrate during prolonged storage at 2-8°C. The crystals can be re-dissolved by swirling the bottle in warm tap water.
General Description
Herpes simplex virus is a member of the herpesvirus group which includes Varicella-zoster, cytomegalovirus and Epstein Barr virus. Replication of the virus occurs within the cell nucleus and is complete upon lysis of the cell. Distinguishing the members of the herpesvirus group can be accomplished by antigenic analysis and definition of biologic properties. In recent times, the subdivision of HSV into specific types has become possible.
It is estimated that at any given time, between 0.65 and 15 percent of all adults may be excreting HSV-1 or HSV-2. Rates of infection appear to be inversely related to socioeconomic status. Only 30-50 percent of adults in higher socioeconomic groups have HSV antibodies while 80-100 percent of the lower socioeconomic groups have specific antibodies.
HSV-1 is primarily spread from oral secretions to other skin areas and recurrent infections occur frequently. On entry into skin sites, herpes simplex virus replicates and instigates a local inflammatory response.
Primary HSV-1 infection is frequently asymptomatic, but occasional minor irritations of the gums, throat and/or eyes occur. Recurrent HSV-1 infections are generally more painful and have serious implications. Occasionally, HSV-1 is believed to be the principal causal agent of herpes simplex encephalitis.
CD has developed a highly sensitive and specific enzyme immunoassay for the detection of Herpes Simplex Type 1 antigen in complex sample matrices derived from both human and veterinary sources. The assay can be completed in less than 2 hr. The test kit incorporates proprietary diluents that are designed to both lyse virions and prevent the development of nonspecific signal derived from complex sample matrix effects and/or the nonspecific adsorption of reactive test components, resulting in improvements in both sensitivity and specificity. In addition, the positive control can be diluted serially to produce a calibration curve for quantitation of HSV-1 antigen. Two- and five-plate test kits are available in a standard photometric detection format.
Standard Curve

Citations


Have you cited DEIAFY104 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

Online Inquiry

Name:
Phone: *
E-mail Address: *
Service & Products Interested: *
Project Description:
Verification Code: * Please input "diagnostics" as verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket