E. Coli GroES, HSP10, recombinant protein from E. coli
Recombinant E.Coli GroES (HSP10)
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
The groES protein contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 1mM DTT and 10% Glycerol.
2-8°C short term, -20°C long term
Escherichia coli serotype O157:H7 is a Shiga toxin-producing pathogen. This serotype has been reported as an etiological agent in sporadic and outbreak cases of haemorrhagic colitis. It is also associated with haemolytic uraemic syndrome. Certain E. coli serotypes other than O157:H7 also produce Shiga toxin. However, the diarrhoea caused by these other serotypes is not usually bloody. Additionally, E. coli serotype O157:H7 does not ferment sorbitol, whereas most other serotypes do. Therefore, if Sorbitol MacConkey Agar is used as a primary screen, the colonies of E. coli serotype O157:H7 appear colourless (non-sorbitol-fermenting colonies [NSFC]) while colonies of the other serotypes appear characteristically pink (sorbitol-fermenting colonies [SFC]).
GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia; E. coli; Escherichia coli; Bacillus coli communis