HIV TAT (full length) (mutation C22), recombinant protein from E. coli
HIV tat (mutated C22) full length protein
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
> 90 % by SDS-PAGE.
Preservative: None Constituents: 20mM NaH2PO4/ Na2HPO4, 2.5% Glycerol, 1.2M Sodium chloride, 50mM Mannitol, 1mM DTT, pH 7.5
Store at -70°C Preservative: None Constituents: 20mM NaH2PO4/ Na2HPO4, 2.5% Glycerol, 1.2M Sodium chloride, 50mM Mannitol, 1mM DTT, pH 7.5
The protein should be reconstituted in apirogenic and sterile dH2O. The reconstituted solution has to be used immediately, since it is not stable in liquid form.
The human immunodeficiency virus (HIV) is a lentivirus (slowly replicating retrovirus) that causes the acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic
The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.
p14; Tat; Tat protein; Transactivating regulatory protein; HIV tat