Lentivirus Titer Kit, HIV-1 p24 ELISA (DEIA3571)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernates
Species Reactivity
Virus
Intended Use
For quantitative detection of HIV-1 p24 Antigen in cell culture supernates for lentiviral particles titration.
Contents of Kit
1. HIV-1 p24 Antibody Coated 96-well Plate ×1
2. Recombinant HIV-1 p24 Standard 100 uL
3. Biotinylated HIV-1 p24 Detection Antibody 10 mL
4. HRP Conjugated Streptavidin-Peroxidase 100 uL
5. Sample Lysis Buffer 5 mL
6. Assay Diluent 25 mL
7. 10x Plate Wash Buffer 25 mL
8. TMB Substrate 10 mL
9. TMB Stop Solution 10 mL
10. Plate Sealer ×2
Storage
All reagents should be stored at 2-8°C except for the detector antibody, and should not be used beyond the expiration date on the label. For more detailed information, please download the following document on our website.
Sensitivity
25 pg/mL

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References


Investigation of binding characteristics of ritonavir with calf thymus DNA with the help of spectroscopic techniques and molecular simulation

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Authors: Kou, Song-Bo; Zhou, Kai-Li; Lin, Zhen-Yi; Lou, Yan-Yue; Wang, Bao-Li; Shi, Jie-Hua; Liu, Ying-Xin

The binding behavior of ritonavir (RTV), a HIV/AIDS protease inhibitor, with ct-DNA was characterized through multiple testing technologies and theoretical calculation. The findings revealed that the RTV-DNA complex was formed through the noncovalent interaction mainly including conventional hydrogen bonds and carbon hydrogen bonds as well as hydrophobic interactions (pi-alkyl interactions). The stoichiometry and binding constant of the RTV-DNA complex were 1:1 and 1.87 x 10(3) M-1 at 298 K, respectively, indicating that RTV has moderate affinity with ct-DNA. The findings confirmed that RTV binds to the minor groove of DNA. The outcomes of CD experiments showed that the binding with RTV changed the conformation of DNA slightly. However, the conformation of RTV had obvious changes after binding to DNA, meaning that the flexibility of RTV molecule played an important role in stabilizing the RTV-DNA complex. Meanwhile, the results of DFT calculation revealed that the RTV and DNA interaction caused the changes in the frontier molecular orbitals, dipole moment and atomic charge distribution of RTV, altering the chemical properties of RTV when it bound to DNA. Communicated by Ramaswamy H. Sarma

Connective Steiner 3-eccentricity index and network similarity measure

APPLIED MATHEMATICS AND COMPUTATION

Authors: Yu, Guihai; Li, Xingfu

For a set S subset of V(G) in a network G, the Steiner distance d(G)(S) of S is the minimum size among all connected subnetworks whose vertex sets contain S. The Steiner k-eccentricity epsilon(kappa) (v) of a vertex v of G is the maximum Steiner distance among all k-vertex set S which contains the vertex v, i.e., s k (v) = max{d(S) vertical bar S subset of V(G), vertical bar S vertical bar = kappa, v is an element of S}. Based on Steiner keccentricity, the connective Steiner k-eccentricity index is introduced. As a newly structural invariant, some properties of the connective Steiner 3-eccentricity index are investigated. Firstly we present an O(n(2))-polynomial time algorithm to calculate the connective Steiner 3-eccentricity index of trees. Secondly some optimal problems among some network classes are discussed. As its application, finally we consider the network similarity measure based on the connective Steiner 3-eccentricity index. By two different methods, we study its advantages. Numerical results show that the measure based on the connective Steiner 3-eccentricity index has more advantages than the ones based on other topological indices (graph energy, Randic index, the largest adjacent eigenvalue, the largest Laplacian eigenvalue). (C) 2020 Elsevier Inc. All rights reserved.

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