For the qualitative determination of HIV-1 p24 in samples.
Contents of Kit
1. Coated microwell strips: 1 plate (96 wells)
2. Positive p24 Control: 10 ng/mL, 0.1 mL
3. Lysis Buffer: 6 mL
4. Detector antibody anti-HIV-1 p24 conjugated to biotin: 12 mL
5. Conjugate: 12 mL
6. Wash Buffer (20x concentrated): 2 Bottles, 60 mL
7. Substrate Solution: 12 mL
8. Stop Solution: 1 N H2SO4, 12 mL
All reagents should be stored at 2-8°C, and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8°C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Opened pouches should be securely resealed by folding over the open end and securing it with adhesive tape I.
The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8°C. It is recommended that Wash buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.
Four samples with different levels of activity were assayed ten times each on three different assays. The results are summarized in the following table.
To determine the sensitivity of the assay, the 0 standard was assayed 20 times. The minimal detectable level was calculated by adding two standard deviations to the mean absorbance for the 0 standard. The minimal detectable level is 1.7 pg/mL.