HHV-6 IgG ELISA Kit (DEIA3565)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

paired serum
Species Reactivity
Intended Use
For the quantitative determination of HHV-6 IgG antibodies in paired serum samples.
Contents of Kit
1. ELISA strips
2. Calibrator
3. Positive control serum
4. Negative control serum
5. Anti-human IgG antibodies
6. Wash buffer
7. Dilution buffer
8. Chromogenic substrate (TMB substrate)
9. Stop solution
Store the kit and the kit reagents at 2-10°C. For longer period make aliquots and keep them at -20°C. Avoid repeated thawing and freezing. For more detailed information, please download the following document on our website.
Performance Characteristics
1. Data from Quality Control test:

2. Validity of the test
The test is valid if:
The background of the reaction (the absorbance of the Dilution buffer) is less than 0.100.
The ratio of Negative control absorbance cut off is lower than 0.9.
The ratio of Positive control absorbance cut-off is higher than 1.1.
The mean absorbance value of Standard 1 is 1.928 for this kit lot, which in the range acceptable OD range:1.400-2.300.
3. Precision of the test
The intra-assay and the inter-assay reproducibility were determined using samples with different absorbance values. In anti-HHV6 IgG-positive samples intra-assay and inter-assay variability coefficients (CV) did not exceed 5% and 15% of mean absorbance values, respectively.

4. Sensitivity and specificity of the test
The sensitivity of the test is 99% and the specificity is 95%. Evaluation was performed by the comparing the HHV-6 IgG ELISA Kit with other commercial ELISA tests.

5. Spiking recovery test
Measured values of recovery test for every Lot are between 80-120% of expected values.
6. Interference
Haemolytic and lipemic samples have no influence on the test results up to concentration of 50 mg/mL of haemoglobin, 5 mg/mL of bilirubin and 50 mg/mL of triglycerides. However, examination of such samples is not recommended.
The sensitivity of the test is 97.9%.
General Description
The kits is intended for serological detection of diseases associated with HHV–6 infection, such as exanthema subitum, acute respiratory illnesses, diarrhoea with fever and febrile seizures in infants, heterophile antibody-negative infectious mononucleosis in children, also interstitial pneumonia, encephalitis, meningitis, hepatitis and aplastic anemia in immunodeficient patients. The presence of IgG anti-HHV-6 antibody reveals the immune status of the patient. Significant rise in anti-HHV-6 IgG antibodies in paired serum samples, taken in acute and convalescent phase of the infection, is indicative of the active infection. The test does not differentiate between HHV6 subtype A and B.


Have you cited DEIA3565 in a publication? Let us know and earn a reward for your research.

Related Products

Customer Reviews

Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket


Horvath, R; Cerny, J; et al. The possible role of human cytomegalovirus (HCMV) in the origin of atherosclerosis. JOURNAL OF CLINICAL VIROLOGY 16:17-24(2000).
Arena, A; Stassi, G; et al. Modulatory effect of HHV-6 on MCP-1 production by human monocytes. MICROBIOLOGICA 25:335-340(2002).

Online Inquiry

Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:
Verification code
Click image to refresh the verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket