Deslorelin High Sensitivity ELISA Kit (DEIA-XYZ69)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
N/A
Intended Use
The Deslorelin EIA Kit is a competitive immunoassay for in vitro quantitative measurement of (Des-Gly10, D-Trp6, Pro-NHEt9)-LHRH (Deslorelin) in serum or plasma.
Contents of Kit
1. EIA buffer concentrate
2. 96-well immunoplate with acetate plate sealer
3. Anti serum (lyophilized powder)
4. Standard (1 ug lyophilized powder)
5. Biotinylated tracer (lyophilized powder)
6. Sreptavidin-HRP
7. TMB substrate solution
8. Stop solution
9. Standard diluent 8ml
10. Datasheet
11. Protocol
Storage
Lyophilized components and standard diluent at a constant -20°C, The remaining components should be stored in the refrigerator (2-4°C)
Performance Characteristics
AVERAGE IC50: 0.4 ng/ml
Detection Range
0-10 ng/ml
Standard Curve

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References


Factors influencing reproductive efficiency and pregnancy in Pura Raza Espanola mares

VETERINARNI MEDICINA

Authors: Akourki, A.; Echegaray, A.; Falceto, M. V.; Mitjana, O.

The aim of this study was to analyse ovarian activity and pregnancy in 140 Pura Raza Espanola mares over a period of two consecutive years. The data were analysed using SPSS software to evaluate the effects of age, category of mares and season on pre-ovulatory follicle size, ovulation rate, interval from foaling to pregnancy, gestation length, incidence of twin pregnancies and pregnancy losses. The results showed that follicular diameter was significantly (P < 0.05) smaller in fillies (3-year-old) and maiden mares than in older and multiparous mares. Within the group of animals treated with gonadotropin hormone hCG, 87.23% ovulated 48 h following administration. Length of gestation varied from 305 to 356 days and all gestation pregnancies of less than 305 days resulted in a non-viable foal. In addition, foaling season affected (P < 0.05) the post-partum conception interval and the length of gestation, which were shorter and longer in the winter foaling, respectively. Regarding the other parameters, mares of this stud exhibited relatively very low rates of double ovulations, twin pregnancies and pregnancy losses. We conclude that age and category of mares and foaling season influence fertility and foaling rates by affecting pre-ovulatory follicle size, pregnancy loss and gestation length.

Use of a gonadotropin releasing hormone agonist implant as an alternative for surgical castration in male ferrets (Mustela putorius furo)

THERIOGENOLOGY

Authors: Schoemaker, N. J.; van Deijk, R.; Muijlaert, B.; Kik, M. J. L.; Kuijten, A. M.; de Jong, F. H.; Trigg, T. E.; Kruitwagen, C. L. J. J.; Mol, J. A.

Surgical castration in ferrets has been implicated as an etiological factor in the development of hyperadrenocorticism in this species due to a castration-related increase in plasma gonadotropins. In search for a suitable alternative, the effect of treatment with the depot GnRH-agonist implant, deslorelin, on plasma testosterone concentrations and concurrent testes size, spermatogenesis, and the typical musky odor of intact male ferrets was investigated. Twenty-one male ferrets, equally divided into three groups, were either surgically castrated, received a slow release deslorelin implant or received a placebo implant. Plasma FSH and testosterone concentrations, testis size and spermatogenesis were all suppressed after the use of the deslorelin implant. The musky odor in the ferrets which had received a deslorelin implant was less compared to the ferrets which were either surgically castrated or had received a placebo implant. These results indicate that the deslorelin implant effectively prevents reproduction and the musky odor of intact male ferrets and is therefore considered a suitable alternative for surgical castration in these animals. (C) 2008 Elsevier Inc. All rights reserved.

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