Sample
serum, plasma, cell lysates, tissue homogenates, and food samples
Intended Use
The Gentamicin ELISA is an immunoassay for the detection of Gentamicin. This test is suitable for the quantitative detection of Gentamicin in samples. FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
Contents of Kit
Box 1 (shipped at room temperature)
1. 96-well Protein Binding Plate: One strip well 96-well plate.
2. Anti-Gentamicin Antibody (500×): One 10 μL vial of anti-Gentamicin Antibody.
3. Secondary Antibody, HRP Conjugate (1000×): One 20 μL vial.
4. Assay Diluent: One 50 mL bottle.
5. 10× Wash Buffer: One 100 mL bottle
6. Substrate Solution: One 12 mL amber bottle.
7. Stop Solution: One 12 mL bottle.
Box 2 (shipped on ice packs)
1. Gentamicin Standard: One 50 μL vial of 150 μM Gentamicin.
2. Gentamicin Conjugate (500×): One 25 μL vial.
3. 100× Conjugate Diluent: One 300 μL vial.
Storage
Upon receipt, store Anti-Gentamicin Antibody (500×), Secondary Antibody HRP Conjugate (1000×), Gentamicin Standard, Gentamicin Conjugate, and 100× Conjugate Diluent at -20°C. Store all the remaining components at 4°C.
General Description
Gentamicin is an antibiotic that has been used to treat various types of bacterial infections such as pelvic inflammatory disease, pneumonia, bone infections, endocarditis, meningitis, sepis, and urinary tract infections. Gentamicin was patented in 1962 and approved to be used medically in 1964.
Gentamicin can be administered topically, intravenously, or by injection into a muscle. Gentamicin is an aminoglycoside that works by blocking synthesis of bacterial proteins resulting in bacterial death.
This antibiotic is made from the bacterium Micromonospora purpurea. As a side effect of treatment, gentamicin can cause inner ear problems (such as hearing loss or poor balance) as well as kidney problems. In addition, gentamicin can cause harm to a developing baby in pregnant women. However, the antibiotic appears to be safe when administered to women who are breastfeeding newborns. Gentamicin is also used by researchers as an antibacterial agent in cell cultures. Gentamicin is a very heat-stable antibiotic, remaining active even after autoclaving, which makes the antibiotic useful in the manufacturing of some types of microbiological growth media.
CD Gentamicin ELISA Kit provides a convenient method for the detection of total Gentamicin in extracts from cells, tissue, serum, plasma, urine, or foods. The total content of gentamicin in unknown samples is determined by comparison with a gentamicin standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 25 nM gentamicin.
Citations
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