Gentamicin ELISA Kit (DEIA047)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
vaccine, cell culture
Species Reactivity
N/A
Intended Use
This kit can be used in quantitative and qualitative analysis of gentamicin residue in vaccine and cell culture.
Contents of Kit
1. Microtiter plate with 96 wells coated with antigen
2. Standard solutions(6x1 mL/bottle)
3. High concentration Gentamicin Standards: (1 mL/bottle) 1μg/mL
4. Enzyme conjugate 12 mL (red cap)
5. Antibody solution 7 mL (green cap)
6. Solution A: 7 mL (white cap)
7. Solution B: 7 mL (red cap)
8. Stop solution: 7 mL (yellow cap)
9. 20xconcentrated wash solution: 40 mL (transparent cap)
10. 2xSample diluent: 50 mL (blue cap)
Storage
2-8°C, 12 months.
Precision
CV of the ELISA kit is less than 10%
Sensitivity
0.1 ng/mL
General Description
Gentamicin residue in the production of biological samples may lead to severe allergic reactions in certain groups. Thus it is strictly controlled in many countries in the world. This kit is a new product for drug residual detection based on ELISA technology, which is rapid, easy-to-use, and sensitive, and can considerably minimize operation errors and work intensity.

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References


Umbelliferone attenuates gentamicin-induced renal toxicity by suppression of TLR-4/NF-kappa B-p65/NLRP-3 and JAK1/STAT-3 signaling pathways

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

Authors: Hassanein, Emad H. M.; Ali, Fares E. M.; Kozman, Magy R.; Abd El-Ghafar, Omnia A. M.

Nephrotoxicity is the most common adverse effect of gentamicin (GNT). This study aimed to investigate the possible nephroprotective effect of umbelliferone (UMB), against GNT-induced nephrotoxicity. Rats were allocated into the control group; UMB group (50 mg/kg/day, P.O. for 15 days); GNT group (100 mg/kg/day, i.p., for 8 days); and GNT + UMB group. By the end of the experimental period, serum creatinine, urea, and uric acid as well as urine KIM-1 and urine albumin/creatinine ratio were evaluated to estimate kidney function. Moreover, tissue samples were collected for assessment of ERK1/2, p-ERK1/2, TLR-4, p38 MAPK, NF-kappa B-p65, NLRP-3, IkB alpha, TNF-alpha, IL-1 beta, JAK1, STAT-3, p-STAT, and cleaved caspase-3. In support, the histopathological examination of renal tissues was performed. UMB improves kidney function through regulation of renal serum biomarkers, with alleviations of histological abrasions induced by GNT. Besides, UMB downregulates renal protein expressions of ERK1/ERK2, TLR-4, and p38MAPK, with subsequent suppression of NF-kappa B-p65/NLRP-3 inflammasome and JAK1/STAT-3 pathways as well as cleaved caspase-3. In parallel, UMB induced IkB alpha upregulation. Collectively, UMB markedly amended all GNT-induced renal changes. These nephroprotective outcomes could be attributed to its ability to impede TLR-4/NF-kappa B-p65/NLRP-3 inflammasome and JAK1/STAT-3 pathways activation, as well as to its anti-inflammatory property.

Theophylline dosing and pharmacokinetics for renal protection in neonates with hypoxic-ischemic encephalopathy undergoing therapeutic hypothermia

PEDIATRIC RESEARCH

Authors: Frymoyer, Adam; Van Meurs, Krisa P.; Drover, David R.; Klawitter, Jelena; Christians, Uwe; Chock, Valerie Y.

Background Theophylline, a non-selective adenosine receptor antagonist, improves renal perfusion in the setting of hypoxia-ischemia and may offer therapeutic benefit in neonates with hypoxic-ischemic encephalopathy (HIE) undergoing hypothermia. We evaluated the pharmacokinetics and dose-exposure relationships of theophylline in this population to guide dosing strategies. Methods A population pharmacokinetic analysis was performed in 22 neonates with HIE undergoing hypothermia who were part of a prospective study or retrospective chart review. Aminophylline (intravenous salt form of theophylline) was given per institutional standard of care for low urine output and/or rising serum creatinine (5 mg/kg intravenous (i.v.) load then 1.8 mg/kg i.v. q6h). The ability of different dosing regimens to achieve target concentrations (4-10 mg/L) associated with clinical response was examined. Results Birth weight was a significant predictor of theophylline clearance and volume of distribution (p < 0.05). The median half-life was 39.5 h (range 27.2-50.4). An aminophylline loading dose of 7 mg/kg followed by 1.6 mg/kg q12h was predicted to achieve target concentrations in 84% of simulated neonates. Conclusions In neonates with HIE undergoing hypothermia, theophylline clearance was low with a 50% longer half-life compared to full-term normothermic neonates without HIE. Dosing strategies need to consider the unique pharmacokinetic needs of this population. Impact Theophylline is a potential renal-protective therapy in neonates with HIE undergoing therapeutic hypothermia; however, the pharmacokinetics and dose needs in this population are not known. Theophylline clearance was low in neonates with HIE undergoing therapeutic hypothermia with a 50% longer half-life compared to full-term normothermic neonates without HIE. As theophylline is advanced in clinical development, dosing strategies will need to consider the unique pharmacokinetic needs of neonates with HIE undergoing therapeutic hypothermia.

Ferguson, Michael W., et al. "The antimalarial drug mefloquine enhances TP53 premature termination codon readthrough by aminoglycoside G418." PloS one 14.5 (2019): e0216423.

Suh, Insoo, et al. "The Efficacy of a Novel Surgical Device in Preventing Intraoperative Wound Contamination in an In Vivo Porcine Model." Journal of Laparoendoscopic & Advanced Surgical Techniques (2017).

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