5 mg of lyophilized Gardiquimod Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Storage and Stability
Gardiquimod is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
- Upon resuspension, prepare aliquots of Gardiquimod and store at -20°C for long term storage. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.
Description
Gardiquimod is a imidazoquinoline compound. Gardiquimod is a Toll-like receptor (TLR)-7 agonist and, like other TLR7 agonists, such as Imiquimod (R837), is an immune response modifier with potent antitumor activity. Gardiquimod has been shown to activate antigen-presenting cells, including dendritic cells (DC) and promote activation of T and natural killer (NK) cells making it a good vaccine adjuvant candidate.
A recent study suggests that Gardiquimod may serve as a vaccine adjuvant to potentiate the efficiency of DC-based tumor immunotherapy. In another study, a tuberculosis vaccine formulated with Gardiquimod was found to protect mice against Mycobacterium tuberculosis when administred intradermally. Furthermore, Velasquez et al. have reported the potential use of Gardiquimod as a mucosal adjuvant for viruslike particles-based vaccines.
Preparation of Sterile Stock Solution (1 mg/ml)
Add 5 ml endotoxin-free physiological water to 5 mg Gardiquimod vial to obtain a solution at 1 mg/ml.
- Mix the solution by pipetting up and down.
Working Concentration
10-100 μg/mouse
Chemical Properties
Formula: C17H23N5O
Molecular weight: 313.4
Solubility: 1 mg/ml in physiological water
