Connexin 43 (Phospho-Ser367) ELISA Kit (DEIA-XYA447)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
2 x 96T
Sample
cultured cells
Species Reactivity
Human, Mouse, Rat
Intended Use
The Connexin 43 (Phospho-Ser367) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Connexin 43 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Connexin 43 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Connexin 43 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL (10x)
3. Quenching Buffer: 24 mL (1x)
4. Blocking Buffer: 50 mL (1x)
5. 10x Wash Buffer: 50 mL (10x)
6. 100x Anti-Connexin 43 (Phospho-Ser367) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-Connexin 43 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x)
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x)
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x)
16. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Kerr, NM; Johnson, CS; et al. Gap junction protein connexin43 (GJA1) in the human glaucomatous optic nerve head and retina. JOURNAL OF CLINICAL NEUROSCIENCE 18:102-108(2011).
Gromova, P; Ralea, S; et al. Kit K641E oncogene up-regulates Sprouty homolog 4 and Trophoblast glycoprotein in interstitial cells of Cajal in a murine model of gastrointestinal stromal tumours. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 13:1536-1548(2009).

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