Sample
Serum, plasma, culture supernatants, cell lysates
Species Reactivity
Human; Rat; Mouse
Intended Use
The Glucagon Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of competitive enzyme immunoassay.
Contents of Kit
1. 96-well plate coated with secondary antibody (Item A): 96 wells (12 strips × 8 wells) coated with secondary antibody.
2. 20× Wash Buffer (Item B): 25 mL.
3. EIA Glucagon Peptide standard, Lyophilized (Item C): 2 vials.
4. Anti-Glucagon Detection Antibody, Lyophilized (Item N): 2 vials.
5. EIA Glucagon 1× Assay Diluent E (Item R): 2 vials, 25 mL/vial. Diluent for both standards and samples including serum or plasma, cell culture media, or other sample types.
6. Biotinylated Glucagon Peptide, Lyophilized (Item F): 2 vials.
7. HRP-streptavidin (Item G): 600 μL of 200× concentrated HRP-conjugated Streptavidin.
8. Glucagon Positive Control Sample, Lyophilized (Item M): 1 vial.
9. TMB Substrate solution (Item H): 12 mL of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution.
10. Stop Solution (Item I): 8 mL of 0.2 M sulfuric acid.
Storage
Standard Glucagon peptide, Biotinylated Glucagon peptide, and Positive Control should be stored at -20°C. Avoid repeated freeze-thaw cycles. The remaining kit components may be stored at 2-8°C. Opened microplate strips and Item N may be store for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. The kit remains active for up to 1 year.
Precision
Intra-Assay: CV <10%
Inter-Assay: CV <15%
Detection Range
1-1,000 pg/mL
Sensitivity
The minimum detectable concentration of Glucagon is 4.77 pg/mL.
Standard Curve
Standard curve(s) is for demonstration only. Standard curve(s) must be run with each assay.
Citations
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