Anti-Gata6 (Internal) polyclonal antibody

The GATA family includes DNA-binding transcription factors which bind to the conserved motif (A/T)GATA(A/G) and play essential roles in cell differentiation as well as adult gene expression regulation along the reproductive axis and embryonic development through regulation of cell proliferation and differentiation. In vertebrates, the GATA gene family consists of six members, likely formed through two major gene duplication events during early vertebrate evolution: GATA1, GATA2, and GATA3 emerged from the initial gene duplication and function in hematopoietic, nervous, and immune systems to control hematopoietic and lymphocyte differentiation.

Figure 1. The GATA proteins with different Zinc finger domains
(Source: Ma M, et al. 2024)

The transcription factor GATA6 from the GATA family includes a conserved DNA-binding domain with two zinc fingers structured as Cys-X₂-Cys-X₁₇-Cys-X₂-Cys. The zinc finger at the C-terminal end binds specifically to the WGATAR (W=A/T, R=A/G) DNA sequence which allows attachment to GATA target sites. The N-terminal zinc finger primarily modulates the DNA-binding capacity of the C-terminal finger by interacting with GATA protein cofactors.

GATA6 regulates liver and pancreatic development through four key stages: Liver specification from endoderm progresses through hepatic bud growth with hepatoblast proliferation/differentiation and morphogenesis which is coordinated by transcription factors from the FoxA and HNF families. During early liver development GATA6 functions as a lineage specification factor and chromatin remodeler while also serving as a pluripotency factor and "pioneer factor" alongside other factors or signaling molecules. The protein has been shown to function as a regulator which maintains the resting state of hepatic stellate cells (HSCs). Beyond transcriptional regulation, GATA6 drives cell fate transitions through lineage commitment, conversion, and transdifferentiation.

Anti-GATA-binding protein 6 (Internal) polyclonal antibody