Fumonisin ELISA Kit (DEIA6848)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
corn, corn meal, corn germ meal, corn gluten meal, soy blend
Species Reactivity
Plant
Intended Use
The Fumonisin Plate Kit is a competitive ELISA for the quantitative analysis of fumonisin in corn, corn meal, corn germ meal, corn gluten meal and corn/soy blend.
Contents of Kit
1. Plate: 96 wells
2. Fumonisin calibrators
3. Fumonisin-HRP Enzyme Conjugate
4. Rabbit anti-Fumonisin antibody
5. Substrate
6. Stop Solution
7. Instructions
Storage
The components of the kit will remain stable through the expiration date indicated on the label and package when stored between 2-8°C. For more detailed information, please download the following document on our website.

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References


The Relationship Analysis on Corn Stalk Rot and Ear Rot According to Fusarium Species and Fumonisin Contamination in Kernels

TOXINS

Authors: Li, Lina; Qu, Qing; Cao, Zhiyan; Guo, Zhengyu; Jia, Hui; Liu, Ning; Wang, Yanhui; Dong, Jingao

Fusarium diseases, including corn root rot, sheath rot, stalk rot, and ear rot are frequently occurring in maize producing areas of China. Fusarium stalk rot and ear rot are the most serious diseases and often occur at the same time, but it is unclear whether there is a correlation between Fusarium composition and disease occurrence. This study was conducted to clarify the relationship between the two diseases. A total of 49 corn stalk rot samples were collected from 15 regions of eight provinces in China from 2016 to 2018. The pathogens were isolated and identified separately from stalks, ear stems, and kernels. The contents of the fumonisins (FB1 and FB2) were detected in kernels. The results showed that the main Fusarium species were found in corn kernels, ear stems and stalks at the same time. The results showed that 1201 strains of Fusarium verticillioides, 668 strains of Fusarium oxysporum, 574 strains of Fusarium graminearum species complex (FGSC), 318 strains of Fusarium equiseti, 95 strains of Fusarium proliferatum, and 40 strains of Fusarium subglutinans were isolated from 1470 corn kernels, 245 ear stems, and 1225 stalks randomly selected from 49 samples. The contamination rate of fumonisins in the 49 samples was 57.1% with an average content of 1.9 mu g/g, of which four samples exhibited higher levels as set by the European Commission (4.0 mu g/g). These results provide a certain association between stalk rot and ear rot and lay a foundation to study the relationships among Fusarium maize diseases.

High contamination levels of deoxynivalenol-induced erythrocyte damage in different models

TRENDS IN FOOD SCIENCE & TECHNOLOGY

Authors: Huang, Yue; Liu, Shuang; Hou, Wei; Xiao, Peng; Chen, Nianjun; Qiu, Pei; Peng, Zhao; Liao, Yuxiao; Wang, Liangliang; Li, Dan; Liu, Liegang; Yang, Wei

Background: Consumptions of DON-contaminated food by farm animals and humans lead to mycotoxicoses. Based on the previous studies, DON may cause the morphological, numerical and functional abnormity of erythrocyte in animals such as pigs, poultry, horses, mice, humans and so on. But at present, there is still a lack of full and systematic discussion of the DON-induced erythrocyte damage. Scope and approach: Therefore, the aim of the present review is to summarize and update the prominent evidences, explore possible mechanisms, put forward preventive measures, and suggest a hypothesis for future research regarding the effects of DON on erythrocyte. Key finding: And conclusions what we summarize are 1) Apart from ruminant, DON can induce erythrocyte damage in animals and there is a threshold level about damage. However, the value of threshold level and the extent of damage are still unclear; 2) DON exerts toxicity on erythrocyte mainly by penetrating the phospholipid bilayers, interacting with the cellular membranes, and phospholipid peroxidation. Most probably, more than one mechanism operates at the same time; 3) The existing methods for preventing damage can be classified as reducing absorption of DON through intestinal tract and blocking mechanisms that DON exerts toxicity on erythrocyte.

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