Human Flt-3L ELISA kit (DEIA5396)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatant, urine
Species Reactivity
Human
Intended Use
The Human Flt-3L ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Flt-3L in serum, plasma, cell culture supernatants and urine.
Contents of Kit
1. Flt-3L Microplate: 96 wells (12 strips x 8 wells) coated with anti-human Flt-3L.
2. Wash Buffer Concentrate (20X): 25 mL of 20X concentrated solution.
3. Standards: 2 vials, recombinant human Flt-3L.
4. Assay Diluent A: 30 mL of animal serum with 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent.
5. Assay Diluent B: 15 mL of 5X concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent.
6. Detection Antibody FLT3L: 2 vial of biotinylated anti-human Flt-3L.
7. HRP-Streptavidin Concentrate: 200 μL of 600X concentrated HRP-conjugated streptavidin.
8. TMB One-Step Substrate Reagent: 12 mL of 3, 3', 5, 5'-tetramethylbenzidine (TMB) in buffered solution.
9. Stop Solution: 8 mL of 0.2 M sulfuric acid.
Storage
May be stored for up to 6 months at 2-8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Precision
Intra-assay: CV<10%
Inter-assay: CV<12%
Sensitivity
1.5 pg/mL

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References


Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells

STEM CELLS INTERNATIONAL

Authors: Barbet, Romain; Peiffer, Isabelle; Hatzfeld, Antoinette; Charbord, Pierre; Hatzfeld, Jacques A.

We present a strategy to identify developmental/differentiation and plasma membrane marker genes of the most primitive human Mesenchymal Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low Density Arrays (TLDA) methodology, we compared the expression of 381 genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs (hES-MSCs), and hMSCs. Analysis of differentiation genes indicated that hES-MSCs express the sarcomeric muscle lineage in addition to the classical mesenchymal lineages, suggesting they are more primitive than hMSCs. Transcript analysis of membrane antigens suggests that IL1R1(low), BMPR1B(low), FLT4(low), LRRC32(low), and CD34 may be good candidates for the detection and isolation of the most primitive hMSCs. The expression in hMSCs of cytokine genes, such as IL6, IL8, or FLT3LG, without expression of the corresponding receptor, suggests a role for these cytokines in the paracrine control of stem cell niches. Our database may be shared with other laboratories in order to explore the considerable clinical potential of hES-MSCs, which appear to represent an intermediate developmental stage between hESCs and hMSCs.

Promoter profiling and coexpression data analysis identifies 24 novel genes that are coregulated with AMPA receptor genes, GRIAs

GENOMICS

Authors: Chong, Allen; Zhang, Zhuo; Choi, Kwok Pui; Choudhary, Vidhu; Djamgoz, Mustafa B. A.; Zhang, Guanglan; Bajic, Vladimir B.

We identified a set of transcriptional elements that are conserved and overrepresented within the promoters of human, mouse, and rat GRIAs by comparing these promoters against a collection of 10,741 gene promoters. Cells regulate functional groups of genes by coordinating the transcriptional and/or posttranscriptional mRNA levels of interacting genes. As such, it is expected that functional groups of genes share the same transcriptional features within their promoters. We found 47 genes whose promoters contain the same combination of transcriptional elements that are overrepresented within the promoters of the GRIA gene family. Coexpressed genes may be transcriptionally coregulated, which in turn suggests that these genes may play complementary roles within a particular functional context. Using microarray expression data, we found 24 (of the 47) genes that share not only a similar promoter profile with GRIAs but also a well-correlated gene expression profile and, thus, we believe these to be coregulated with GRIAs. (c) 2006 Elsevier Inc. All rights reserved.

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