Intended Use
The Fasciola hepatica IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiqualitative determination of IgG-class antibodies to Fasciola in serum and plasma (EDTA-, or heparin -plasma).
Contents of Kit
1. Microtiter wells, 12 × 8 (break apart) strips, 96 wells; Wells coated with Fasciola antigen. (incl. 1 cover foil)
2. Sample Diluent *, 1 vial, 60 mL, ready to use, colored yellow; pH 7.2 ± 0.2.
3. Pos. Control *, 1 vial, 2.0 mL, ready to use; colored yellow, red cap.
4. Neg. Control *, 1 vial, 2.0 mL, ready to use; colored yellow, yellow cap.
5. Cut-off Control*, 1 vial, 2.0 mL, ready to use; colored yellow, black cap.
6. Enzyme Conjugate *, 1 vial, 20 mL, ready to use, colored red, antibody to human IgG conjugated to horseradish peroxidase.
7. Substrate Solution, 1 vial, 14 mL, ready to use, Tetramethylbenzidine (TMB).
8. Stop Solution, 1 vial, 14 mL, ready to use, contains 0.2 mol/L H2SO4, Avoid contact with the stop solution. It may cause skin irritations and burns.
9. Wash Solution *, 1 vial, 30 mL (20× concentrated for 600 mL), pH 6.5 ± 0.1.
* contain non-mercury preservative
Storage
When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Opened reagents must be stored at 2 °C to 8 °C. Microtiter wells must be stored at 2 °C to 8 °C. Once the foil bag has been opened, care should be taken to close it tightly again.
Opened kits retain activity for two months if stored as described above.
COA
Publication 
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