FTO (intracellular; mouse) EIA Kit (DEIA5082)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell lysates
Species Reactivity
Mouse
Intended Use
CD's FTO (intracellular; mouse) EIA Kit is an immunometric assay which can be used to measure FTO in mouse cell lysates.
Contents of Kit
1. Anti-FTO (mouse) Precoated 96-Well Strip Plate, 1 plate
2. Wash Buffer Concentrate (10x), 1 vial/50 mL
3. Dilution Buffer (5X), 1 vial/50 mL
4. Detection Antibody, 1 vial/12 mL
5. Anti-Rabbit IgG/HRP Conjugate (100x), 1 vial/150 μL
6. FTO (mouse) EIA Standard, 1 vial
7. Substrate Solution, 1 vial/12 mL
8. Stop Solution, 1 vial/12 mL
9. Quality Control Sample, 1 vial
10. Lysis Buffer Concentrate (10x), 1 vial/12 mL
Storage
This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box. Reagents must be stored at 4°C when not in use. Reagents must be brought to room temperature before use. Do not expose reagents to temperatures greater than 25°C. Diluted Wash Solution may be stored at room temperature for up to one month.
Precision
Intra-assay Precision
(%): 2.2-3.7

Inter-assay Precision
(%): 3.9-6.3
Detection Range
0-10 ng/mL
Sensitivity
20 pg/mL

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References


The role of chlorine atom on the binding between acrylonitrile derivatives and fat mass and obesity-associated protein

JOURNAL OF MOLECULAR RECOGNITION

Authors: Bai, Ning; Gan, Ya; Li, Xitong; Gao, Shuting; Yu, Wenquan; Wang, Ruiyong; Chang, Junbiao

In this work, seven acrylonitrile derivatives were selected as potential inhibitors of fat and obesity-related proteins (FTO) by the aid of fluorescence spectroscopy, ultraviolet visible spectroscopy, molecular docking, and cytotoxicity methods. Results show that the interaction between 3-amino-2-(4-chlorophenyl)-3-phenylacrylonitrile (1a) and FTO was the strongest among these derivatives. Thermodynamic analysis and molecular modeling show that the main force between 1a and FTO is hydrophobic interaction. The cytotoxicity test showed that the IC50 value of 1a was 46.64 mu mol/L, which indicated 1a had the smallest IC50 value and had the best inhibitory effect on the proliferation of leukemia K562 cells among the seven derivatives. Both our previous results and this work show that chlorine atoms play important role in the binding of small molecules and FTO. This work brings new information for the study of FTO inhibitors.

Glucose Oxidase/Nano-ZnO/Thin Film Deposit FTO as an Innovative Clinical Transducer: A Sensitive Glucose Biosensor

FRONTIERS IN CHEMISTRY

Authors: Asrami, Padideh Naderi; Azar, Parviz Aberoomand; Tehrani, Mohammad Saber; Mozaffari, Sayed Ahmad

In the present research, a new biocompatible electrode is proposed as a rapid and direct glucose biosensing technique that improves on the deficiencies of fast clinical devices in laboratory investigations. Nano-ZnO (nanostructured zinc oxide) was sputtered by reactive direct current magnetron sputtering system on a precovered fluorinated tin oxide (FTO) conductive layer. Spin-coated polyvinyl alcohol (PVA) at optimized instrumental deposition conditions was applied to prepare the effective medium for glucose oxidase enzyme (GOx) covalent immobilization through cyanuric chloride (GOx/nano-ZnO/PVA/FTO). The electrochemical behavior of glucose on the fabricated GOx/nano-ZnO/PVA/FTO biosensor was investigated byI-Vtechniques. In addition, field emission scanning electron microscopy and electrochemical impedance spectroscopy were applied to assess the morphology of the modified electrode surface. TheI-Vresults indicated good sensitivity for glucose detection (0.041 mA per mM) within 0.2-20 mM and the limit of detection was 2.0 mu M. We believe that such biodevices have good potential for tracing a number of biocompounds in biological fluids along with excellent accuracy, selectivity, and precise analysis. The fast response time of the fabricated GOx/nano-ZnO/PVA/FTO biosensor (less than 3 s) could allow most types of real-time analysis.

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