Intended Use
This kit is a competitive enzyme-labeled immunoassay and can be used in quantitative analysis of ferulic acid in wheat.
Contents of Kit
1.Microtiter plate with 96 wells coated with coupling antigen
2.Spiking standard 25ug/mL, 200ul
3.Standard Dilution Buffer, 35mL
4.20× Sample Dilution Buffer, 35mL
5.Antibody Solution, 7mL
6.Enzyme Conjugate, 12mL
7.Substrate Solution, 2×6mL
8.Stop Solution, 7mL
9.20× Wash Solution, 50mL
10.Empty brown tubes, 7
11.Instruction
Storage
1. All reagents should be stored at 2°C to 8°C for stability.
2. Before using, bring all components to room temperature (20-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
3. The Substrate and Spiking standard is light-sensitive and should be protected from direct sunlight or UV sources.
Precision
Intra-Assay: CV<10%
Inter-Assay: CV<10%
Detection Range
0-243 ng/ml
Citations
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Ferulic acid (FA) is a hydroxycinnamic acid that belongs to a class of aromatic acids called phenylpropanoids. It is derived from cinnamic acid, commonly found in fruits and vegetables such as tomatoes, sweet corn, and rice bran. It has broad therapeutic effects on a variety of diseases including diabetes, cancer, cardiovascular and neurodegenerative diseases.
Figure 1. Structure of FA.
(Source: Srinivasan, M. et al., 2007)
One of the notable properties of FA is its powerful antioxidant activity, which has a positive impact on human health. This makes it effective against free radicals, including reactive oxygen species (ROS). As a potent free radical scavenger, FA has been approved in some countries as a food additive to prevent lipid peroxidation. Animal studies and in vitro experiments suggest that FA may have a pro-apoptotic effect on cancer cells, leading to their death. It has shown potential as an antineoplastic agent, particularly against breast and liver cancer. Additionally, some studies suggest that FA may protect against cancer caused by exposure to carcinogens such as benzopyrene and 4-nitroquinoline-oxide.
The FA ELISA Kit provides researchers, scientists, and professionals with a valuable tool to study the presence and concentration of FA in wheat. The kit includes all the necessary components and reagents, along with a detailed protocol, ensuring ease of use and accurate results.
Alternative Names
Ferulic acid
Ferulic acid ELISA
Ferulic acid (FA) ELISA
FA ELISA
Ferulic acid (FA) ELISA Kit
FA ELISA Kit
ELISA Kit for Ferulic acid (FA)
References
1. Srinivasan M, et al. Ferulic acid: therapeutic potential through its antioxidant property. Journal of Clinical Biochemistry and Nutrition. 2007, 40(2): 92-100.
Antioxidant properties of ferulic acid and its possible application
Skin Pharmacology and Physiology
Authors: Zduńska K, Dana A, Kolodziejczak A, et al.
Abstract
Ferulic acid has low toxicity and possesses many physiological functions (anti-inflammatory, antioxidant, antimicrobial activity, anticancer, and antidiabetic effect). It has been widely used in the pharmaceutical, food, and cosmetics industry. Ferulic acid is a free radical scavenger, but also an inhibitor of enzymes that catalyze free radical generation and an enhancer of scavenger enzyme activity. Ferulic acid has a protective role for the main skin structures: keratinocytes, fibroblasts, collagen, elastin. It inhibits melanogenesis, enhances angiogenesis, and accelerates wound healing. It is widely applied in skin care formulations as a photoprotective agent, delayer of skin photoaging processes, and brightening component. Nonetheless, its use is limited by its tendency to be rapidly oxidized.
Chemistry, natural sources, dietary intake and pharmacokinetic properties of ferulic acid: A review
Food Chemistry
Authors: Zhao Z, Moghadasian M H.
Abstract
Ferulic acid (FA) is an abundant dietary antioxidant which may offer beneficial effects against cancer, cardiovascular disease, diabetes and Alzheimer's disease. The impact of FA on health depends on its intake and pharmacokinetic properties. In this article, the literature pertaining to chemistry, natural sources, dietary intake and pharmacokinetic properties of FA is critically reviewed. High levels of FA are found in both free and bound forms in vegetables, fruits, cereals, and coffee. We have estimated that consumption of these foods may result in approximately 150–250 mg/day of FA intake. FA can be absorbed along the entire gastrointestinal tract and metabolized mainly by the liver. The absorption and metabolism of FA seem to be dose dependent at least in experimental settings. Further pharmacokinetic and pharmacodynamic studies are required to characterize the impact of FA on human health.
COA
Certificate of Analysis
