This kit can be used in quantitative and qualitative analysis of erythromycin residue in tissue(muscle), cell supernatant, vaccine, water, honey and milk.
Contents of Kit
1.Microtiter plate with 96 wells coated with antigen 2.Erythromycin standard solutions. (1ml×6 bottles) 0 ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb 3.Spiking standard control 1ppm (1ml) 4.Enzyme conjugate (7ml)……..…...……….red cap 5.Antibody solution (7ml) ….………………green cap 6.Substrate solution(6ml*2)…………………...……..…red cap 7.Stop solution (7ml) …………………...…yellow cap 8.20× Concentrated wash solution (50ml) …………transparent cap 9.2× Concentrated extraction solution (50ml) ………………………..… blue cap
Erythromycin is a macrolide antibiotic, which is applied as antibacterial and anti-mycoplasma infective. Strict MRLs have been established since this drug may lead to serious side effect in certain groups. This kit is a new product for drug residual detection based on ELISA technology, which only costs 1h in each operation and can considerably minimize operation errors and work intensity compared with instrumental analysis.
Citations
Publication ()
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Background
Erythromycin, belonging to the macrolide antibiotic class, is extensively employed in veterinary and human medicine. Erythromycin can be added to feed to promote animal growth and prevent diseases. However, its residues in food products or environmental samples can pose potential health risks and raise concerns regarding antibiotic resistance. Therefore, a reliable and precise method for detecting erythromycin residues is essential for ensuring food safety and environmental monitoring.
Figure 1. Results obtained from analysis of different honey samples by ELISA and residual erythromycin in different studied regions diagram. (Source: Mehrabi, A. et al., 2023)
Our Enzyme Immunoassay Kits for Erythromycin are designed to detect erythromycin residue in tissue (muscle), cell supernatant, vaccine, water, honey, and milk. These kits have wide applications across different sectors such as food and drug administration and research institutions. They meet the stringent requirements of consumers for food quality and provide efficient experimental data for scientific researchers.
1. Mehrabi A, et al. ELISA evaluation of erythromycin residues in honey samples collected from different areas of Qazvin, Iran. Journal of Chemical Health Risks. 2023,3(4): 647-652.
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References
The molecular mechanism of peptide-mediated erythromycin resistance
The macrolide antibiotic erythromycin binds at the entrance of the nascent peptide exit tunnel of the large ribosomal subunit and blocks synthesis of peptides longer than between six and eight amino acids. Expression of a short open reading frame in 23 S rRNA encoding five amino acids confers resistance to erythromycin by a mechanism that depends strongly on both the sequence and the length of the peptide. In this work we have used a cell-free system for protein synthesis with components of high purity to clarify the molecular basis of the mechanism. We have found that the nascent resistance peptide interacts with erythromycin and destabilizes its interaction with 23 S rRNA. It is, however, in the termination step when the pentapeptide is removed from the peptidyl-tRNA by a class 1 release factor that erythromycin is ejected from the ribosome with high probability. Synthesis of a hexa- or heptapeptide with the same five N-terminal amino acids neither leads to ejection of erythromycin nor to drug resistance. We propose a structural model for the resistance mechanism, which is supported by docking studies. The rate constants obtained from our biochemical experiments are also used to predict the degree of erythromycin resistance conferred by varying levels of resistance peptide expression in living Escherichia coli cells subjected to varying concentrations of erythromycin. These model predictions are compared with experimental observations from growing bacterial cultures, and excellent agreement is found between theoretical prediction and experimental observation.
COA
Publication 
Figure 1. Results obtained from analysis of different honey samples by ELISA and residual erythromycin in different studied regions diagram.
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