E. coli Heat-labile enterotoxin B (DAG3923)

E. coli Heat-labile enterotoxin B, recombinant protein from P. fluorescens Datasheet

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Product Overview
Recombinant Escherichia coli Heat-labile enterotoxin B
Nature
Recombinant
Tag/Conjugate
Unconjugated
Purity
greater than 95%, by SDS-CGE under reducing conditions Endotoxin Level: less than 1000 EU/mg of protein by LAL method
Format
Lyophilized in phosphat buffered saline, pH 7.2 Product Molecular Mass: 11.7 kDa, as monomer, 58.6 kDa as pentamer
Buffer
Tris/NaCl buffer
Storage
2-8°C short term, -20°C long term
Introduction
Escherichia coli serotype O157:H7 is a Shiga toxin-producing pathogen. This serotype has been reported as an etiological agent in sporadic and outbreak cases of haemorrhagic colitis. It is also associated with haemolytic uraemic syndrome. Certain E. coli serotypes other than O157:H7 also produce Shiga toxin. However, the diarrhoea caused by these other serotypes is not usually bloody. Additionally, E. coli serotype O157:H7 does not ferment sorbitol, whereas most other serotypes do. Therefore, if Sorbitol MacConkey Agar is used as a primary screen, the colonies of E. coli serotype O157:H7 appear colourless (non-sorbitol-fermenting colonies [NSFC]) while colonies of the other serotypes appear characteristically pink (sorbitol-fermenting colonies [SFC]).
Antigen Description
Heat-labile enterotoxin is an oligomeric protein complex secreted by certain virulent strains of E.coli and is one of the pathogenic agents responsible for the symptoms of traveler\'s diarrhea. The holotoxin consists of a single A subunit surrounded by fi
Keywords
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia; E. coli; Escherichia coli; Bacillus coli communis

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