Endoglin ELISA Kit (DEIA-XYA991)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell lysates, serum, plasma
Species Reactivity
Human
Intended Use
The Human Endoglin ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Endoglin concentrations within any experimental sample including cell lysates, serum and plasma.
Contents of Kit
1. Microstrips Coated w/ Capture Antibody: 12 x 8-Well Microcstrips
2. Protein Standard: Lyophilized (25 ng), Red
3. Biotinylated Detection Antibody: Lyophilized, Yellow
4. 400x Streptavidin-HRP: 30 μL, Blue
5. Wash Buffer (10x): 50 mL, Clear
6. Assay Diluent: 50 mL, Clear
7. Ready-to-Use Substrate: 12 mL, Brown
8. Stop Solution: 12 mL, Clear
9. Adheive Plate Sealers: 4 Seets
10. Technical Manual: 1 Manual
Storage
4°C/6 Months
Detection Range
125-8000 pg/mL
Sensitivity
The Human Endoglin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Endoglin proteins within the range of 125-8000 pg/mL.
Standard Curve

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References


Artificial Intelligence-Based Smart Engineering Education

SENSORS AND SMART STRUCTURES TECHNOLOGIES FOR CIVIL, MECHANICAL, AND AEROSPACE SYSTEMS 2020

Authors: Ouyang, Fan; Jiao, Pengcheng; Alavi, Amir H.

Artificial intelligence has the capacity to open new opportunities and potentials for engineering education. Artificial intelligence in education has undergone several paradigmatic shifts in its brief history. This study investigates the advent, development and future trends of artificial intelligence-based smart engineering education (AIED-Eng). Particular focus is placed on major paradigms in AIED-Eng including leaner-receiver, learner-partner and learner-center. The artificial intelligence techniques applied to these paradigms are evaluated. Computer-based tools enabling the engineering education paradigms are summarized. Further discussion is presented about the key role of artificial intelligence in improving smart engineering education as a guidance for future learning, teaching and design processes.

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

Authors: Wang, Yong; Zhang, Ding; Zhou, Fang; Zhou, Meijun; Li, Qiujie; Chen, Jingyu; Yang, Jun

Vascular development is determined by the sequential expression of specific genes, which can be studied by performing in situ hybridization assays in zebrafish during different developmental stages. To investigate the role of endoglin(eng) in vessel formation during the development of hereditary hemorrhagic telangiectasia (HHT), morpholino-mediated targeted knockdown of eng in zebrafish are used to study its temporal expression and associated functions. Here, whole-mount in situ RNA hybridization (WISH) is employed for the analysis of eng and its downstream genes in zebrafish embryos. Also, tube formation assays are performed in HHT patient-derived induced pluripotent stem cell-differentiated endothelial cells (iPSC-ECs; with eng mutations). A specific signal amplifying system using the whole amount In Situ Hybridization - WISH provides higher resolution and lower background results compared to traditional methods. To obtain a better signal, the post-fixation time is adjusted to 30 min after probe hybridization. Because fluorescence staining is not sensitive in zebrafish embryos, it is replaced with diaminobezidine (DAB) staining here. In this protocol, HHT patient-derived iPSC lines containing an eng mutation are differentiated into endothelial cells. After coating a plate with basement membrane matrix for 30 min at 37 degrees C, iPSC-ECs are seeded as a monolayer into wells and kept at 37 degrees C for 3 h. Then, the tube length and number of branches are calculated using microscopic images. Thus, with this improved WISH protocol, it is shown that reduced eng expression affects endothelial progenitor formation in zebrafish embryos. This is further confirmed by tube formation assays using iPSC-ECs derived from a patient with HHT. These assays confirm the role for eng in early vascular development.

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