Enterovirus IgG ELISA Kit (DEIA-XY121)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, EDTA plasma, heparin plasma, citrate plasma
Species Reactivity
Human
Intended Use
Enzyme immunoassay (ELISA) for the determination of human IgG antibodies directed against Enteroviruses in serum or plasma (EDTA, citrate, heparin).
Contents of Kit
1. Break apart microtiter test strips each with eight antigen coated single wellsB: 12 pieces
2. Standard serum (ready-to-use): 2 x 2 mL
3. Negative control serum (ready-to-use): 2 mL
4. Anti-human IgG conjugate (ready-to-use): 13 mL
5. Washing solution concentrate (sufficient for 1000 mL): 33.3 mL
6. Dilution buffer: 2 x 50 mL
7. Stopping solution: 15 mL
8. Substrate (ready-to-use): 13 mL
9. Quality control certificate with standard curve and evaluation table: 1 page
Storage
2-8°C
Precision
Intra-Assay Precision: 2.1%-2.2%
Inter-Assay Precision: 8.2%-10.9%
Sensitivity
>92%

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References


Optical characteristics of Cu 1-x Fe x O thin films prepared via electrophoretic deposition technique

PHYSICA B-CONDENSED MATTER

Authors: Al-Shuayfani, Shatha A.; Loucif, Aicha; Gassoumi, Malek; Shadad, Maged N.; Amer, Mabrook S.

Cu1-xFexO (x = 0, 2, 4, and 6 at. %) thin films were deposited on fluorine-doped tin oxide (FTO) substrates using the electrophoretic deposition (EPD) technique. Before the EPD process, Cu1-xFexO powders were synthesized using the solid-state method. In this method, the powders are mechanically milled up to 9 h followed by annealing at 450 x25e6;C for 2 h. The XRD patterns reveal that all the investigated films are pure polycrystalline phase CuO, with monoclinic structure and without any traces of Fe or its oxides. The FESEM morphology observations of all the films confirm the constitution of grains whose average size varies from 100 to 250 nm. Moreover, increase in Fe dopant amount led to a change in the number and size of the particle agglomerations and the number of porosities. The effect of Fe dopant concentration on the optical constants of CuO films was studied using a UV-Vis spectrophotometer. The estimated band gap values shown blue-shifted from 1.23 eV for pure to 1.49 eV for Cu0.94Fe0.06O. Furthermere, the refractive index and Urbach energy values were determined, and the correlation between the results obtained with XRD data and FESEM morphology is discussed.

Superior antibacterial activity against seed-borne plant bacterial disease agents and enhanced physical properties of novel green synthesized nanostructured ZnO using Thymbra spicata plant extract

CERAMICS INTERNATIONAL

Authors: Sahin, B.; Soylu, S.; Kara, M.; Turkmen, M.; Aydin, R.; Cetin, H.

An easy and eco-friendly approach using Thymbra spicata var. spicata L. (TS) plant extract was developed for the formation of nanostructured ZnO. TS aqueous leaf extract was used for the green synthesis of nanostructured ZnO via the Successive ionic layer adsorption and reaction (SILAR) method. Electron microscope images exhibit the morphological adjustments of the samples with respect to change in TS concentration in the growth solution. The nanostructured ZnO grown by SILAR was observed to be polycrystalline with hexagonal crystal structure. The optical energy bandgap value of the samples varies from 3.21 to 3.09 eV as the content of TS increases from 2.5 to 5.0%. Also, the effect of TS additive to ZnO on electrical properties was investigated. It was determined by Van der Pauw measurements that TS contribution to ZnO significantly increased electrical resistance. In addi-tion, impedance analyzes of the produced films were carried out in the frequency range of 20Hz -1 MHz. Nyquist plots showed the single semicircle for all samples, and the values of capacitance and resistance were calculated. Its antibacterial activities was investigated against economically important Gram-positive (Clavibacter michiganensis subsp. Michiganensis) and negative (Pseudomonas syringae pv. Phaseolicola, Pseudomonas cichorii and Pectobacterium carotovorum subsp. Carotovorum) seed-borne plant bacterial disease agents by using paper disc diffusion assay for the first time. In vitro laboratory screenings of green synthesized nanostructured ZnO have given encouraging results, indicating their potential use in the management of seed-borne bacterial diseases.

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