EV-71 IgM DOT-ELISA Kit (DEIA435)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
6T, 30T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
ThisEV-71 IgM DOT-ELISA is an enzyme-linked immuno-filtration method for qualitative determination of IgM-class antibodies to human enterovirus-71(EV71) in serum or plasma samples. The assay is intended to be used in clinical laboratories for early diagnosis and management of patients related to infection with EV71.
Contents of Kit
1. EV71 IgM Immuno-filtration Devices
2. Sample treatment solution
3. Sample treatment bottles
4. Antigen reagent
5. HRP-conjugate
6. Wash Buffer
7. Chromogen Solution
8. Stop Solution
Storage
Store the kit reagents at 2-8°C. Avoid repeated thawing and freezing. For more detailed information, please download the following document on our website.

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References


Umami-enhancing effect of typical kokumi-active gamma-glutamyl peptides evaluated via sensory analysis and molecular modeling approaches

FOOD CHEMISTRY

Authors: Yang, Juan; Huang, Yuran; Cui, Chun; Dong, Hao; Zeng, Xiaofang; Bai, Weidong

The umami-enhancing effect of typical kokumi-active gamma-glutamyl peptides was verified by sensory evaluation. To investigate the umami-enhancing molecular mechanism of the peptide on monosodium glutamate (MSG) taste, a novel hypothetical receptor, taste type 1 receptor 3 (T1R3)-MSG complex, was constructed. These peptides demonstrated strong interactions with T1R3-MSG. Moreover, four amino acid residues, Glu-301, Ala-302, Thr-305, and Ser-306, were critical in ligand-receptor interactions. In detail, gamma-Glu-gamma-Glu-Val (gamma-E-gamma-EV) readily interacts with T1R3 through hydrogen bonds and hydrophobic interactions. While gamma-E-gamma-EV did not bind to MSG, gamma-Glu-Val (gamma-EV) and gamma-Glu-Leu (gamma-EL) showed high binding affinity to MSG and interacted with T1R3 through hydrophobic bonds suggesting that the interactions between dipeptides and T1R3-MSG were weaker than tripeptides. These results demonstrated that kokumi-active gamma-glutamyl peptides could enhance the umami taste of MSG, and exhibit synergistic effects in activating T1R3. This study provides a theoretical reference for interactions between the novel umami-enhancing substances and umami receptor.

Extracellular vesicle-shuttled miRNAs: a critical appraisal of their potential as nano-diagnostics and nano-therapeutics in type 2 diabetes mellitus and its cardiovascular complications

THERANOSTICS

Authors: Prattichizzo, Francesco; Matacchione, Giulia; Giuliani, Angelica; Sabbatinelli, Jacopo; Olivieri, Fabiola; de Candia, Paola; De Nigris, Valeria; Ceriello, Antonio

Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease causing the development of a large range of cardiovascular (CV) complications. Lifestyle changes and pharmacological therapies only partially halt T2DM progression, and existing drugs are unable to completely suppress the increased CV risk of T2DM patients. Extracellular vesicles (EV)s are membrane-coated nanoparticles released by virtually all living cells and are emerging as novel mediators of T2DM and its CV complications. As a matter of fact, several preclinical models suggest a key involvement of EVs in the initiation and/or progression of insulin resistance, beta-cell dysfunction, diabetic dyslipidaemia, atherosclerosis, and other T2DM complications. In addition, preliminary findings also suggest that EV-associated molecular cargo, and in particular the miRNA repertoire, may provide with useful diagnostic and/or prognostic information for the management of T2DM. Here, we review the latest findings showing that EV biology is altered during the entire trajectory of T2DM, i.e. from diagnosis to development of CV complications. We also critically highlight the potential of this emerging research field, by describing both preclinical and clinical observations, and the limitations that must be overcome to translate the preclinical findings into the development of EV-based nano-diagnostic and/or nano-therapeutic tools. Finally, we summarize how two lifestyle changes known to prevent or limit T2DM, i.e. diet and exercise, affect EV number and composition, with a focus on the possible role of EVs contained in food in shaping metabolic responses, a promising approach still in its infancy.

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