ENA-6 Profile ELISA Kit (DEIA1864)

Regulatory status: For research use only, not for use in diagnostic procedures.

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serum, plasma
Species Reactivity
Intended Use
The ENA-6 Profile ELISA Kit is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for semi-quantitative determination of IgG class autoantibodies directed against the extractable nuclear antigens SS-A (Ro), SS-B (La), Sm, RNP/Sm, Scl-70 and Jo-1 in human serum or plasma.
Contents of Kit
1. Divisible microplate
2. Anti-ENA controls
3. Positive Control
4. Negative Control
5. Cut-off Calibrators
6. Sample buffer
7. Enzyme conjugate solution
8. TMB substrate solution
9. Stop solution
10. Wash solution
Store the kit at 2-8°C. For more detailed information, please download the following document on our website.


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Analysis of the perception of engineering students regarding sustainability


Authors: Rampasso, I. S.; Anholon, R.; Silva, D.; Cooper Ordonez, R. E.; Santa-Eulalia, L. A.; Quelhas, O. L. G.; Filho, W. Leal; Granada Aguirre, L. F.

This research aims to evaluate the engineering students' perception regarding sustainability. For this, a survey was developed based on sustainability parameters from a detailed analysis of the Global Reporting Initiative (GRI) and the Brazilian Institute of Corporate Governance (BICG). The parameters were initially divided into seven groups: Financial and Productivity Aspects (FPA); Concern With Employees (CWE); Support for Local Communities (SLC); Ethical and Corporate Governance Issues (ECI); Environmental Aspects (ENA); Sustainable Aspects in the Operations Network (SON); Customers, Development of New Products and Services (CPS). The survey was conducted with engineering undergraduate students from two Brazilian universities. The data were analyzed through structural equation modeling technique, namely the PLS-SEM algorithm. The collected 162 answers enabled the validation of the model tested, and showed that the students, in general, do not consider SLC and CWE when they are analyzing sustainability. Additionally, the most important construct was the CPS. This is an exploratory study and we believe that these findings may contribute to expand the debate about the sustainability insertion in engineering courses, helping educators in their didactic activities. There were not found similar studies in the literature, which highlight the originality of the research. The statistical validation of the results and the contribution to expand the debate regarding sustainability in engineering education justify the value of this study. (C) 2019 Elsevier Ltd. All rights reserved.

Increase in water temperature increases acute toxicity of sumithion causing nuclear and cellular abnormalities in peripheral erythrocytes of zebrafish Danio rerio


Authors: Shahjahan, Md; Rahman, Mohammad Shadiqur; Islam, S. M. Majharul; Uddin, Md Helal; Al-Emran, Md

Global warming and indiscriminate usages of pesticides are notable concern to all. The present study has been conducted to evaluate the effects of high temperature on acute toxicity of sumithion in adult zebrafish. A 2-day renewal bioassay system was used to determine the 96 h LC50 value of sumithion at three temperature regimes, such as 25 degrees C, 30 degrees C, and 35 degrees C. Blood glucose (mg/dL) level was measured in control (0.0 mg/L) and low concentration (1.0 mg/L) of sumithion during the determination of LC50 in three temperature conditions. In addition, micronucleus (MN), erythrocytic nuclear abnormalities (ENA), and erythrocytic cellular abnormalities (ECA) tests were performed in the blood erythrocytes. The 96 h LC50 value of sumithion for zebrafish was significantly lower at 35 degrees C, which indicates that the toxicity of sumithion increases at higher temperature. Blood glucose level was significantly increased by sumithion in all temperature conditions, while it was significantly higher in the highest (35 degrees C) temperature compared to the lowest (25 degrees C) temperature in both control and sumithion-treated fish. Similarly, frequencies of MN, ENA, and ECA were elevated by sumithion in all temperature conditions, whereas it was significantly raised in the highest (35 degrees C) temperature compared to the lowest (25 degrees C) temperature in both control and sumithion treated fish. With increasing temperature in exposure to sumithion, dissolved oxygen decreased significantly, whereas free CO2 increased significantly. On the other hand, no distinct changes were observed in pH and total alkalinity during the experimental period. Therefore, it can be inferred that increasing temperature enhances the toxicity of sumithion in the zebrafish.

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