Human EHD1 peptide

Name: Human EHD1 peptide
SKU: DAG-P1555
Rating: 5 (1 reviews)

Synthetic Human EHD1 peptide

COA

SDS

Datasheet

Summary

Specificity

Highly expressed in testis.

Nature

Synthetic

Tag/Conjugate

Unconjugated

SequenceSimilarities

Contains 1 EF-hand domain.Contains 1 EH domain.

Alternative Names

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Purity

70 - 90% by HPLC.

Format

Liquid

Preservative

None

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Information available upon request.

Entrez Gene ID

10938

Antigen Description

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Function

ATP binding; GTP binding; GTPase activity; Rab GTPase binding; calcium ion binding; protein binding;

Synonyms

EHD1; EH-domain containing 1; PAST; PAST1; H-PAST; HPAST1; EH domain-containing protein 1; testilin; PAST homolog 1;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

The Endocytic Recycling Regulatory Protein EHD1 Is Required for Ocular Lens Development

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Authors: Arya, Priyanka; Rainey, Mark; Bhattacharyya, Sohinee; Mohapatra, Bhopal; Kuracha, Murali; Band, Vimla; Govindarajan, Venkatesh; Band, Hamid

Abstract

GRAF1 forms a complex with MICAL-L1 and EHD1 to cooperate in tubular recycling endosome vesiculation

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY

Authors: Cai, Bishuang; Xie, Shuwei; Caplan, Steve; Naslavsky, Naava

Abstract

The biogenesis of tubular recycling endosomes (TREs) and their subsequent vesiculation after cargo-sorting has occurred, is essential for receptor and lipid recycling to the plasma membrane. Although recent studies have implicated the C-terminal Eps15 Homology Domain (EHD) protein, EHD1, as a key regulator of TRE vesiculation, additional proteins involved in this process have been largely uncharacterized. In the present study, we identify the GTPase Regulator Associated with Focal adhesion kinase-1 (GRAF1) protein in a complex with EHD1 and the TRE hub protein, Molecules Interacting with CasL-Like1 (MICAL-L1). Over-expression of GRAF1 caused vesiculation of MICAL-L1-containing TRE, whereas GRAF1-depletion led to impaired TRE vesiculation and delayed receptor recycling. Moreover, co-addition of purified EHD1 and GRAF1 in a semi-permeabilized cell vesiculation assay produced synergistic TRE vesiculation. Overall, based on our data, we suggest that in addition to its roles in clathrin-independent endocytosis, GRAF1 synergizes with EHD1 to support TRE vesiculation.