EBV P23 (DAG3928)

EBV P23, recombinant protein from E. coli

Product Overview
Recombinant Epstein-Barr Virus p23
EBV- p23 protein is greater than 95% pure as determined by 10% PAGE (coomassie staining). EBV-p23 was purified by proprietary chromatographic technique.
2-8°C short term, -20°C long term
The Epstein–Barr virus (EBV), also called human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. It is best known as the cause of infectious mononucleosis (glandular fever). It is also associated with particular forms of cancer, such as Hodgkin's lymphoma, Burkitt's lymphoma, nasopharyngeal carcinoma, and central nervous system lymphomas associated with HIV. There is evidence that infection with the virus is associated with a higher risk of certain autoimmune diseases, especially dermatomyositis, systemic lupus erythematosus, rheumatoid arthritis and multiple sclerosis. Infection with EBV occurs by the oral transfer of saliva
Antigen Description
The Epstein Barr virus (EBV) is a human herpes virus 4 (HHV4) that infects and establishes latency in B-lymphocytes. Primary infection leads to a life-long past infection which is normally asymptomatic. EBV expresses a number of genes, however, Epstein Ba
EBV p23; HHV-4 p23; HHV4 p23 protein; EBV p23 protein; EBV; Epstein Barr virus p23 protein; HHV4 p23; Human Herpesvirus 4; Probable capsid protein VP23; Protein BDLF1; VP23; Herpesviridae; Lymphocryptovirus; EBV VP23; Epstein–Barr virus VP23


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EBV P23 [GST] (DAG511)

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Efficient evaluation of humoral immune responses by the use of serum pools


Authors: Sternbaek, Louise; Draborg, Anette H.; Nielsen, Christoffer T.; Jacobsen, Soren; Iversen, Line V.; Troelsen, Lone; Theander, Elke; Houen, Gunnar

Background: Collection and testing of individual serum samples are often used in research to gain knowledge about e.g. the humoral response against bacteria or virus. This is a valid but time-consuming method and might be a waste of valuable serum samples for inefficient research. So far, no study has considered using serum pools as a quick and efficient screening method to confirm or deny hypotheses. Methods: We created serum pools from four different patient groups (systemic lupus erythematosus n = 85, rheumatoid arthritis n = 77, Sjogren's syndrome n = 91, systemic sclerosis n = 66) and one healthy control group (n = 67). Each serum pool was analyzed using three well-known immunoassays: enzyme-linked immunosorbent assay (ELISA), line blot, and immunofluorescence microscopy (anti-nuclear antibody (ANA) screening). The presence of Epstein -Barr virus (EBV) EA/D-, EBNA-1-, VCA p23-, and gp350-directed antibodies was used to validate serum pools as an efficient tool for further investigations by comparison to previous findings in this area. Results: The presence of EBV EA/D-, EBNA-1-, VCA p23-, and gp350-directed antibodies in each pool was consistent within the obtained ELISA and line blot results, as increased titers of IgG against the four antigens were found in all patient serum pools and also in individual sera regarding gp350. These results correspond to previous findings on individual samples from patients with these diseases. The presence of ANAs was observed in all four patient serum pools and not in the HC pool by both line blots and immunofluorescence microscopy, which corresponds with the expectations and further corroborate the application of serum pools for screenings. Conclusion: We developed and validated the use of serum pools that reliably and rapidly can confirm or deny hypotheses, which enables a more efficient research concentrating on the most evident factors. (C) 2017 Elsevier B.V. All rights reserved.

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