Regulatory status: For research use only, not for use in diagnostic procedures.

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serum, plasma
Species Reactivity
Intended Use
Quantitative and qualitative tests for detection of human anti-Epstein-Barr Virus antibodies in serum or plasma.
Contents of Kit
1.Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96) MTP, 1 frame, the coating material is inactivated, 12
2.Standard serum (ready-to-use) STD Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide, colouring: Amaranth O, 2 x 2 ml
3.Negative control serum (ready-to-use) NEG Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, colouring: Lissamin green V, 2 ml
4.Anti-human-IgG-conjugate (ready-to-use) APC Anti-human-IgG from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution, preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane, 13 ml
5.Washing solution concentrate (sufficient for 1 litre) WASH Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide, 33.3 ml
6.Dilution buffer DILB Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide 0.01 g/l Bromphenol blue sodium salt, 2 x 50 ml
7.Stopping solution STOP 1.2 N sodium hydroxide, 15 ml
8.Substrate (ready-to-use) pNPP Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!), 13 ml
9.Quality control certificate with standard curve and evaluation table INFO (quantification of antibodies in IU/ml or U/ml), 1
General Description
Epstein-Barr Virus (EBV), a DNA virus, is a member of the Human Herpesvirus group and is pathogenic for humans.
EBV transmission takes place primarily via the saliva of infected individuals. Transfer of the virus via blood, blood products and bone marrow transplants have also been reported, but this mode of transmission is comparatively rare.
On primary infection, the cells of the salivary gland are infected first. At this stage of infection respiratory symptoms are very common. Due to the subsequent infection of B cells in the adjacent lymphoid tissue, the virus spreads throughout the body. Infection of B cells results in a polyclonal stimulation of lymphoproliferation which is normally controlled by the immune system. In the later course of infection, high fever, splenomegaly, lymphadenitis, thrombocytopenia and hepatitis may be observed. The disease resulting from primary infection is called infectious mononucleosis (IM) or glandular fever. Since viral transmission principally happens by oral contact, the primary infection has also been called "kissing disease". In rare cases acute mononucleosis may progress to a chronic disease, and reactivation of EBV has been observed in immunosuppressed patients.
EBV is closely associated with nasopharyngeal carcinoma, and Burkitt lymphoma (BL), is at least partly correlated with EBV. This B cell tumor of monoclonal origin is endemically clustered in tropical regions of Africa and Asia. The geographical distribution of BL correlates with that of malaria. Since it is believed that infections with Plasmodium have some influence on the immune system, it is suggested that malaria might be an important cofactor for the development of Burkitt lymphoma in these regions.
Due to the complex structure of the virus, the tight regulation of the viral life cycle, and the appearance of latent or productive infections, the antibody response seen after EBV infection may be quite complex and therefore difficult to interpret.


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MDM2's dual mRNA binding domains co-ordinate its oncogenic and tumour suppressor activities


Authors: Gnanasundram, Sivakumar Vadivel; Malbert-Colas, Laurence; Chen, Sa; Fusee, Leila; Daskalogianni, Chrysoula; Muller, Petr; Salomao, Norman; Fahraeus, Robin

Cell growth requires a high level of protein synthesis and oncogenic pathways stimulate cell proliferation and ribosome biogenesis. Less is known about how cells respond to dysfunctional mRNA translation and how this feeds back into growth regulatory pathways. The Epstein-Barr virus (EBV)-encoded EBNA1 causes mRNA translation stress in cis that activates PI3K delta. This leads to the stabilization of MDM2, induces MDM2's binding to the E2F1 mRNA and promotes E2F1 translation. The MDM2 serine 166 regulates the interaction with the E2F1 mRNA and deletion of MDM2 C-terminal RING domain results in a constitutive E2F1 mRNA binding. Phosphorylation on serine 395 following DNA damage instead regulates p53 mRNA binding to its RING domain and prevents the E2F1 mRNA interaction. The p14Arf tumour suppressor binds MDM2 and in addition to preventing degradation of the p53 protein it also prevents the E2F1 mRNA interaction. The data illustrate how two MDM2 domains selectively bind specific mRNAs in response to cellular conditions to promote, or suppress, cell growth and how p14Arf coordinates MDM2's activity towards p53 and E2F1. The data also show how EBV via EBNA1-induced mRNA translation stress targets the E2F1 and the MDM2 - p53 pathway.

CutaneousEpstein-Barrvirus-associated smooth muscle tumor in immunosuppression


Authors: Matin, Rubeta N.; Ieremia, Eleni

A 36-year-old renal transplant recipient presented 15 months post-transplantation with a cutaneous spindle cell neoplasm with features of smooth muscle differentiation treated with local excision. 1.4 years later, a magnetic resonance imaging liver scan with gadolinium demonstrated multiple bilobar enhancing hepatic lesions, in keeping with metastases. A core biopsy revealed morphological appearances similar to the previous cutaneous spindle cell neoplasm. Epstein-Barr virus early RNA (EBER) in situ hybridization demonstrated strong diffuse staining of both cutaneous and liver tumor cells for EBER indicative of Epstein-Barr virus (EBV) infection. This is a rare presentation of multifocal EBV-associated smooth muscle tumors first presenting in the skin in an adult renal transplant recipient, which, despite being multifocal and involving the liver, may confer a better prognosis than predicted.

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