E. coli RuvB (DAG-P2720)

E. coli RuvB (full length), recombinant protein from E. coli Datasheet

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Product Overview
E. coli RuvB full length protein
Cellular Localization
Cell Membrane, Cytoplasmic and Nuclear
> 90 % by SDS-PAGE.purified by methods such as chromatography
Preservative: None Constituents: 50% Glycerol, 5mM Beta mercaptoethanol, 2mM EDTA, 100mM Sodium chloride, 10mM Tris HCl, pH 7.5
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Preservative: None Constituents: 50% Glycerol, 5mM Beta mercaptoethanol, 2mM EDTA, 100mM Sodium chloride, 10mM Tris HCl, pH 7.5
Escherichia coli; commonly abbreviated E. coli) is a gram-negative, facultatively anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harml
Antigen Description
In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC,RuvAB is uncoupled from RuvC and DSBs may be prevented.
49 kDa TATA box binding protein interacting protein; 49 kDa TBP interacting protein; 54 kDa erythrocyte cytosolic protein; ATP dependent DNA helicase, component of RuvABC resolvasome; DNA helicase p50; ECP 54; Holliday junction ATP dependent DNA helicase


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