E. coli G, U mismatch-specific DNA glycosylase (DAG-P2308)

E. coli G, U mismatch-specific DNA glycosylase (aa 1 - 168), recombinant protein from E. coli Datasheet

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Product Overview
E. coli G/U mismatch-specific DNA glycosylase full length protein
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
19 kDa
Cellular Localization
Cytoplasmic
Purity
>95% by SDS-PAGE .Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. This antigen has been 0.2 μM filtered.
Format
Liquid
Buffer
pH: 8.00Constituents: 0.02% PMSF, 0.02% Beta mercaptoethanol, 0.32% Tris HCl, 50% Glycerol
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. pH: 8.00Constituents: 0.02% PMSF, 0.02% Beta mercaptoethanol, 0.32% Tris HCl, 50% Glycerol
Introduction
Escherichia coli; commonly abbreviated E. coli) is a gram-negative, facultatively anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harml
Antigen Description
Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.
Keywords
Double strand specific uracil glycosylase; Mismatch specific uracil DNA glycosylase; MUG; Xanthine DNA glycosylase; ygjF; E. coli G/U mismatch-specific DNA glycosylase; Escherichia coli G/U mismatch-specific DNA glycosylase

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