Dog haptoglobin reference serum (DAGA-627)

Dog haptoglobin reference serum, native protein

Canine, Dog
Alternative Names
Dog; Haptoglobin; Serum
Batch dependent - please inquire should you have specific requirements
0.1% Sodium Azide
Frozen -20°C
Antigen Description
Haptoglobin (Hp) is an acute phase protein that binds the free hemoglobin (Hb), thus preventing iron loss and renal damage. Hp also has antioxidative and immunomodulatory properties.The haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia. In intravascular hemolysis, free hemoglobin will be released into circulation and hence haptoglobin will bind the hemoglobin.


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Effects of dietary extruded linseed andLactobacillus acidophiluson growth performance, carcass traits, plasma lipoprotein response, and caecal bacterial populations in broiler chicks


Authors: Gheorghe, A.; Lefter, N. A.; Idriceanu, L.; Ropota, M.; Habeanu, M.

The study aimed to assess the effects of dietary extruded linseed (ELS) andLactobacillus acidophilus(LA) on performance, carcass traits, immune organ weights, plasma lipoprotein response, and caecal bacteria populations in broiler chickens. A total of 648 one-day-old chicks were divided into six groups in a 3 x 2 factorial arrangement consisting of three ELS levels (0, 6 and 12%) without or with probioticL. acidophilus(0 and 20 g ton(-1)feed). Each group had six replicates (18 birds/pen). Results showed no significant effects of ELS level nor LA supplementation on growth performance (body weight gain, feed intake, and feed conversion) of broilers during the overall period (1-42 days). The production efficiency factor increases (p = .045) with the LA addition. There were no effects of ELS level nor probiotic addition on carcase traits and immune organ weights, except for decreases in abdominal fat percentage (p = .027, respectivelyp = .035). The dietary ELS level significantly correlated with a decrease in the plasma total cholesterol (TC;p = .003), triglycerides, and very-low-density lipoprotein concentrations (p = .020), while the LA addition significantly correlated with a decrease (p = .015) in the plasma TC. The LA addition lowers the caecal pH (p = .007),Staphylococcusspp. andE. colicounts (p < .0001), and increase the caecalLactobacillusspp. and lactobacilli:E. coliratio (p < .0001). In conclusion, the use of ELS up to 12% supplemented withL. acidophilusin broiler chicks diet had a positive effect on health status, decreasing the abdominal fat deposition, plasma lipids and the caecal pathogen bacteriaE. coli.

Short communication: Effects of transition milk and milk replacer supplemented with colostrum replacer on growth and health of dairy calves


Authors: Van Soest, B.; Cullens, F.; VandeHaar, M. J.; Nielsen, M. Weber

Transition milk (TM, defined here as the second through fourth milkings after calving) supplies additional fat, protein, and immunoglobulins to the calf compared with milk replacer at industry-suggested feeding rates (similar to 14% solids). Our objective was to determine whether 9 feedings of TM on d 2 through 4 of life increase the growth rate and overall health of calves. Holstein heifer calves on a commercial farm were randomly assigned to 1 of 3 diets (n = 35/diet): milk replacer (MR; Purina Warm Front BOV MOS Medicated Milk Replacer, St. Louis, MO), TM, or a 50:50 blend of MR and colostrum replacer (MCR; Alta HiCal Colostrum Powder Replacer, the Saskatoon Colostrum Company Ltd., Saskatoon, SK, Canada). The TM was harvested from Holstein cows on the farm, pooled, and pasteurized at 71.7 degrees C for 15 s. Nutrient composition on a dry matter basis of TM was 25.9% fat, 41.8% protein, and 14% solids; MR was 10.3% fat, 27.8% protein, and 14% solids; and MCR was 14.6% fat, 38.6% protein, and 15% solids. All calves received IgG-enriched colostrum replacer for the first 2 feedings after birth. Subsequently, calves were fed 1.9 L of MR, TM, or MCR 3 times per day for 3 d (starting on d 2). After initial diets ended, calves were fed and managed similarly. Body weights (d 1, 7, 14, 21, and 56), blood samples (d 1, 7, 14, and 21), and daily health scores (scale of 0 to 3, with 0 representing normal or healthy and 3 representing severe symptoms or ill) were collected through weaning at 56 d. All except 1 calf achieved successful transfer of passive immunity, with serum IgG values greater than 10.0 mg/mL. From birth through weaning, calves fed TM and MCR gained 3 kg more total body weight than those fed MR (34.3, 34.3, and 31.3 kg, respectively). Increased metabolizable energy (using NRC 2001 recommendations) in TM accounts for 0.68 kg of the increased gain compared with MR. Treatment did not alter health scores for ears, eyes, or feces. Haptoglobin concentrations were lower in TM and MCR than in MR calves (4.63, 3.62, and 7.54 mu g/mL, respectively), whereas lipopolysaccharide binding protein concentrations were not different. In conclusion, compared with MR alone, feeding TM or MR with colostrum replacer for 3 d increased growth rate of calves throughout the preweaning period.

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