Dog NGAL reference serum (DAGA-629)

Dog NGAL reference serum, native protein

Specificity
Canine, Dog
Nature
Native
Tag/Conjugate
Unconjugated
Alternative Names
Dog; NGAL; Serum
Procedure
None
Format
Liquid
Concentration
Batch dependent - please inquire should you have specific requirements
Size
1ml
Preservative
0.1% Sodium Azide
Storage
Frozen -20°C
Antigen Description
Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), is a protein that in humans is encoded by the LCN2 gene. NGAL is involved in innate immunity by sequestrating iron that in turn limits bacterial growth. It is expressed in neutrophils and in low levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts. NGAL is used as a biomarker of kidney injury.
Keywords
Dog;NGAL;Serum

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References


Construction of succinimide group substituted polythiophene polymer functionalized sensing platform for ultrasensitive detection of KLK 4 cancer biomarker

SENSORS AND ACTUATORS B-CHEMICAL

Authors: Aydin, Elif Burcu; Aydin, Muhammet; Sezginturk, Mustafa Kemal

A novel impedimetric immunosensor based on conjugated succinimide group substituted polythiophene polymer (SucS-PThi) modified indium tin oxide (ITO) electrode was fabricated for ultra-sensitive and ultra-selective determination of kallikrein 4 (KLK 4) antigen in diluted human serum. The basic reason for the usage of SucS-PThi polymer was that its rich effective functional groups and biocompatibility. KLK 4, an important prostate biomarker was the target analyte and anti-KLK 4 antibodies immobilized electrode was the diagnostic tool for KLK 4 antigen detection. The fabricated biosensor was characterized by employing electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX), fourier transform-infrared (FTIR) and Raman spectroscopy. Under optimized conditions, a linear concentration range from 0.04 pg/mL to 30 pg/mL and a low detection limit (LOD) of 12.2 fg/mL were obtained and the LOD was lower than of most of the existing techniques, illustrating its feasibility for practical application. Moreover, the developed sensing system combined the outstanding properties in terms of selectivity, sensitivity, repeatability, reproducibility and reusability, without requiring for labor - intensive labeling stages. In addition, it was successfully utilized for accurate quantification of KLK 4 antigen in human serum samples.

Protective effects of Glycyrrhiza glabra supplementation against methotrexate-induced hepato-renal damage in rats: An experimental approach

JOURNAL OF ETHNOPHARMACOLOGY

Authors: Chauhan, Prerna; Sharma, Himanshu; Kumar, Uma; Mayachari, Anand; Sangli, Gopalkrishna; Singh, Surender

Ethnopharmacological relevance: In traditional medicine, Glycyrrhiza glabra, commonly known as liquorice, is known to possess promising pharmacological properties including anti-oxidative, anti-inflammatory, gastro, hepato and nephro-protective activities. Aim: The present study investigated the protective effects of Glycyrrhiza glabra rhizome extract (GGE) on MTX-induced hepato-renal damage in Wistar albino rats. Materials and methods: Rats were pre-treated with GGE (100, 200 or 400 mg/kg) from day 1 to 15 and administered MTX (20 mg/kg) on day 4. Methotrexate-induced hepato-renal damage was assessed by serum toxicity biomarkers (AST, ALT, BUN and creatinine), oxidative stress estimation (MDA, GSH, SOD, CAT, peroxidase and glutathione reductase), interleukins profiling (TNF-alpha, IL-1 beta, IL-6 and IL-12), tissue histopathology and immunohistochemical (caspase-3 and NFkB) examination. Results: MTX induced hepato-renal damage resulted in elevated serum levels of AST, ALT, BUN and creatinine, increased pro-inflammatory cytokines concentration and accumulation of MDA and reduced levels of GSH, SOD, CAT, peroxidase and glutathione reductase. Conversely, co-treatment with GGE dose-dependently ameliorated oxidative stress, serum interleukins, hepato-renal toxicity biomarkers (p < 0.001), preserved tissue architecture and downregulated both caspase-3 and NFkB expression in hepato-renal tissue. Conclusion: The above results suggested that GGE can alleviate MTX-induced hepato-renal damage by decreasing oxidative stress and suppressing the ensuing activation of pro-apoptotic and pro-inflammatory pathways.

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